Autoimmunity to a cornea-associated stromal antigen in patients with Mooren's ulcer

John D Gottsch, S. H. Liu, J. B. Minkovitz, D. F. Goodman, M. Srinivasan, W. J. Stark

Research output: Contribution to journalArticle

Abstract

Purpose. To purify and characterize a cornea-associated antigen (CO-Ag) and to determine antibody levels to CO-Ag in patients with Mooren's ulcer. Method. Standard ion exchange and gel filtration chromatographies were used to isolate and purify CO-Ag from crude bovine stromal extracts. The serum of a patient with Mooren's ulcer, containing a high level of antibodies directed against CO-Ag, was used to monitor isolation procedures. Using this newly purified CO-Ag, an enzyme-linked immunoabsorbent assay was used to detect the presence of antibodies to CO-Ag in the sera of other patients with Mooren's ulcer. Results. CO-Ag was purified to apparent homogeneity from bovine corneal stromal extracts by a series of ion exchange chromatographies and gel filtration. Polyacrylamide gel electrophoresis showed that CO-Ag was a tetramer with a molecular weight of 30,000 d that may dissociate under denaturing conditions into a monomer of 7000 d. Strong indirect immunofluorescent staining was demonstrated of the stroma by guinea pig anti- CO-Ag antibody. A statistically significant difference in the level of specific antibodies to CO-Ag between patients with Mooren's ulcer and controls was found (P <0.001). The antibody level was elevated in patients with Mooren's ulcer (mean antibody level, 0.58 ± 0.13) compared with the controls (mean antibody level, 0.22 ± 0.04). Conclusion. These results suggest that an autoantigen exists in the corneal stroma that reacts with serum antibodies from patients with Mooren's ulcer. The availability of a purified corneal antigen could facilitate the diagnosis and define the pathogenetic mechanisms in Mooren's ulcer.

Original languageEnglish (US)
Pages (from-to)1541-1547
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number8
Publication statusPublished - 1995

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Keywords

  • autoantibodies
  • cornea-associated antigen
  • Mooren's ulcer
  • stromal antigen

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Gottsch, J. D., Liu, S. H., Minkovitz, J. B., Goodman, D. F., Srinivasan, M., & Stark, W. J. (1995). Autoimmunity to a cornea-associated stromal antigen in patients with Mooren's ulcer. Investigative Ophthalmology and Visual Science, 36(8), 1541-1547.