Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition

Máire E. Doyle, Lori Boggs, Robert Attia, Lauren R. Cooper, Daniel R. Saban, Cuong Q. Nguyen, Ammon B. Peck

Research output: Contribution to journalArticle

Abstract

Sjögren's syndrome (SjS) is a human autoimmune disease characterized by exocrine dysfunction resulting from chronic autoimmune attack primarily against the lacrimal and/or salivary glands. Although, we previously established a good correlation between SjS in humans and autoimmune exocrinopathy in NOD/LtJ mice an in-depth evaluation of lacrimal gland disease in the NOD/LtJ mouse has remained limited. This leaves a major gap in our understanding of the dacryoadenitis/keratoconjunctivitis sicca in this model. Here we characterize the development of the autoimmune dacryoadenitis in NOD/LtJ and NOD.B10-H2 b mice in comparison with age- and sex-matched nonautotmmune CD1 mice. We observed a decline in tear production beginning at 8 weeks of age in both NOD/LtJ and NOD.B10-H2b mice, continuing throughout the 40 to 46 weeks studied. This correlated with a quantifiable increase in mixed T- and B-lymphocyte infiltrations in the extraorbital lacrimal glands. In addition, temporal differences in tear protein expression between NOD/LtJ and CD1 mice were identified using two-dimensional gel electrophoresis and tandem mass spectrometry. Thus, using this model we can identify potentially important pathophysiological mechanisms of the autoimmune attack and possible diagnostic markers for development of SjS-associated dacryoadenitis.

Original languageEnglish (US)
Pages (from-to)1224-1236
Number of pages13
JournalAmerican Journal of Pathology
Volume171
Issue number4
DOIs
StatePublished - Oct 2007

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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