Autoantigenic determinants on human thyroglobulin. II. Determinants recognized by autoantibodies from patients with chronic autoimmune thyroiditis compared to autoantibodies from healthy subjects

Herbert S. Bresler, C. Lynne Burek, William H. Hoffman, Noel R. Rose

Research output: Contribution to journalArticle

Abstract

Using a panel of 20 well-characterized mouse monoclonal antibodies (mAbs) to native human thyroglobulin (Tg), the fine specificities of autoantibody responses in human autoimmune thyroiditis patients were compared to naturally occurring autoantibodies from healthy individuals. The antigenic determinants recognized by the human autoantibodies were assessed using competitive binding assays in which murine mAbs were inhibited by human autoantibodies. All determinants of human Tg recognized by the mouse were also responded to by the human subjects. Autoantibodies from adult and juvenile patients with chronic lymphocytic thyroiditis recognized principally 10 of 19 determinants defined by the panel of murine mAbs. The mAbs which define these 10 determinants do not react with Tg from seven other mammalian species; therefore, autoantibody responses in individuals with thyroiditis are directed primarily to species specific determinants of human Tg. These same determinants on human Tg were the principal ones recognized by the mouse following immunization. Particular patterns of fine specificities were not inherited from parents by probands or their siblings, even if identical twins. Naturally occurring Tg autoantibodies from healthy subjects recognized mainly thyroxine-containing cross-reactive determinants.

Original languageEnglish (US)
Pages (from-to)76-86
Number of pages11
JournalClinical Immunology and Immunopathology
Volume54
Issue number1
DOIs
StatePublished - 1990

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Autoimmune Thyroiditis
Thyroglobulin
Autoantibodies
Healthy Volunteers
Monoclonal Antibodies
Thyroiditis
Hashimoto Disease
Competitive Binding
Monozygotic Twins
Chronic Thyroiditis
Thyroxine
Population Groups
Siblings
Epitopes
Immunization
Parents

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Autoantigenic determinants on human thyroglobulin. II. Determinants recognized by autoantibodies from patients with chronic autoimmune thyroiditis compared to autoantibodies from healthy subjects. / Bresler, Herbert S.; Lynne Burek, C.; Hoffman, William H.; Rose, Noel R.

In: Clinical Immunology and Immunopathology, Vol. 54, No. 1, 1990, p. 76-86.

Research output: Contribution to journalArticle

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