Autoantibodies to thyroid tissue sediment detected by the antiglobulin consumption test

Joseph H. Kite, Robert C. Brown, Noel R. Rose

Research output: Contribution to journalArticle

Abstract

The antiglobulin consumption test (AGCT) has been adapted to the demonstration of autoantibodies in the serum of patients with chronic thyroiditis. Human thyroid tissue was homogenized and washed 15 times with buffered saline so that the supernatant was free of detectable thyroglobulin and serum. The lyophilized tissue sediment was then used in the AGCT. Positive reactions were obtained with 23 of 64 human thyroiditis sera. No reaction occurred with normal human sera. The results of the consumption tests were compared with hemagglutination tests employing tanned red blood cells coated with soluble human thyroid extracts. A direct correlation between the 2 titers was seen in most but not all cases. The reactions observed in the AGCT appeared to be species and organ specific and were not due to isoantibodies. Absorption of thyroiditis serum with purified human thyroglobulin removed part of the consumption titer. This finding suggested that some of the thyroglobulin was either tightly bound to the insoluble tissue components or was present in an insoluble form. It also points to the possible presence of antibodies to other insoluble antigens of the thyroid tissue sediment. When compared with lyophilized human thyroid tissue sediment or lyophilized thyroid tissue microsomes, frozen microsomal fractions gave additional consumption in tests with thyroiditis serum absorbed with thyroglobulin. Boiling of the microsomal fraction reduced consumption with absorbed serum which indicated the thermolability of the insoluble tissue antigen(s).

Original languageEnglish (US)
Pages (from-to)179-194
Number of pages16
JournalThe Journal of Laboratory and Clinical Medicine
Volume59
Issue number2
StatePublished - Feb 1962
Externally publishedYes

Fingerprint

Coombs Test
Autoantibodies
Anti-Idiotypic Antibodies
Thyroid Gland
Sediments
Tissue
Thyroglobulin
Thyroiditis
Serum
Antigens
Hemagglutination Tests
Isoantibodies
Microsomes
Boiling liquids
Blood
Demonstrations
Erythrocytes
Cells
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Autoantibodies to thyroid tissue sediment detected by the antiglobulin consumption test. / Kite, Joseph H.; Brown, Robert C.; Rose, Noel R.

In: The Journal of Laboratory and Clinical Medicine, Vol. 59, No. 2, 02.1962, p. 179-194.

Research output: Contribution to journalArticle

Kite, Joseph H. ; Brown, Robert C. ; Rose, Noel R. / Autoantibodies to thyroid tissue sediment detected by the antiglobulin consumption test. In: The Journal of Laboratory and Clinical Medicine. 1962 ; Vol. 59, No. 2. pp. 179-194.
@article{530bf445688648b5b9561f132bbd1ba2,
title = "Autoantibodies to thyroid tissue sediment detected by the antiglobulin consumption test",
abstract = "The antiglobulin consumption test (AGCT) has been adapted to the demonstration of autoantibodies in the serum of patients with chronic thyroiditis. Human thyroid tissue was homogenized and washed 15 times with buffered saline so that the supernatant was free of detectable thyroglobulin and serum. The lyophilized tissue sediment was then used in the AGCT. Positive reactions were obtained with 23 of 64 human thyroiditis sera. No reaction occurred with normal human sera. The results of the consumption tests were compared with hemagglutination tests employing tanned red blood cells coated with soluble human thyroid extracts. A direct correlation between the 2 titers was seen in most but not all cases. The reactions observed in the AGCT appeared to be species and organ specific and were not due to isoantibodies. Absorption of thyroiditis serum with purified human thyroglobulin removed part of the consumption titer. This finding suggested that some of the thyroglobulin was either tightly bound to the insoluble tissue components or was present in an insoluble form. It also points to the possible presence of antibodies to other insoluble antigens of the thyroid tissue sediment. When compared with lyophilized human thyroid tissue sediment or lyophilized thyroid tissue microsomes, frozen microsomal fractions gave additional consumption in tests with thyroiditis serum absorbed with thyroglobulin. Boiling of the microsomal fraction reduced consumption with absorbed serum which indicated the thermolability of the insoluble tissue antigen(s).",
author = "Kite, {Joseph H.} and Brown, {Robert C.} and Rose, {Noel R.}",
year = "1962",
month = "2",
language = "English (US)",
volume = "59",
pages = "179--194",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Autoantibodies to thyroid tissue sediment detected by the antiglobulin consumption test

AU - Kite, Joseph H.

AU - Brown, Robert C.

AU - Rose, Noel R.

PY - 1962/2

Y1 - 1962/2

N2 - The antiglobulin consumption test (AGCT) has been adapted to the demonstration of autoantibodies in the serum of patients with chronic thyroiditis. Human thyroid tissue was homogenized and washed 15 times with buffered saline so that the supernatant was free of detectable thyroglobulin and serum. The lyophilized tissue sediment was then used in the AGCT. Positive reactions were obtained with 23 of 64 human thyroiditis sera. No reaction occurred with normal human sera. The results of the consumption tests were compared with hemagglutination tests employing tanned red blood cells coated with soluble human thyroid extracts. A direct correlation between the 2 titers was seen in most but not all cases. The reactions observed in the AGCT appeared to be species and organ specific and were not due to isoantibodies. Absorption of thyroiditis serum with purified human thyroglobulin removed part of the consumption titer. This finding suggested that some of the thyroglobulin was either tightly bound to the insoluble tissue components or was present in an insoluble form. It also points to the possible presence of antibodies to other insoluble antigens of the thyroid tissue sediment. When compared with lyophilized human thyroid tissue sediment or lyophilized thyroid tissue microsomes, frozen microsomal fractions gave additional consumption in tests with thyroiditis serum absorbed with thyroglobulin. Boiling of the microsomal fraction reduced consumption with absorbed serum which indicated the thermolability of the insoluble tissue antigen(s).

AB - The antiglobulin consumption test (AGCT) has been adapted to the demonstration of autoantibodies in the serum of patients with chronic thyroiditis. Human thyroid tissue was homogenized and washed 15 times with buffered saline so that the supernatant was free of detectable thyroglobulin and serum. The lyophilized tissue sediment was then used in the AGCT. Positive reactions were obtained with 23 of 64 human thyroiditis sera. No reaction occurred with normal human sera. The results of the consumption tests were compared with hemagglutination tests employing tanned red blood cells coated with soluble human thyroid extracts. A direct correlation between the 2 titers was seen in most but not all cases. The reactions observed in the AGCT appeared to be species and organ specific and were not due to isoantibodies. Absorption of thyroiditis serum with purified human thyroglobulin removed part of the consumption titer. This finding suggested that some of the thyroglobulin was either tightly bound to the insoluble tissue components or was present in an insoluble form. It also points to the possible presence of antibodies to other insoluble antigens of the thyroid tissue sediment. When compared with lyophilized human thyroid tissue sediment or lyophilized thyroid tissue microsomes, frozen microsomal fractions gave additional consumption in tests with thyroiditis serum absorbed with thyroglobulin. Boiling of the microsomal fraction reduced consumption with absorbed serum which indicated the thermolability of the insoluble tissue antigen(s).

UR - http://www.scopus.com/inward/record.url?scp=50549162405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50549162405&partnerID=8YFLogxK

M3 - Article

C2 - 14456554

AN - SCOPUS:50549162405

VL - 59

SP - 179

EP - 194

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 2

ER -