Autoantibodies against B23, a nucleolar phosphoprotein, occur in scleroderma and are associated with pulmonary hypertension

Research output: Contribution to journalArticle

Abstract

Objective. To determine whether the abundant nucleolar phosphoprotein B23 is a target of autoantibodies in scleroderma, and to examine the clinical phenotype associated with these antibodies. Methods. Ninety-two randomly selected scleroderma sera were screened by enzyme-linked immunosorbent assay against recombinant human B23. Demographic, clinical, and serologic parameters associated with B23 autoantibody status were examined. Results. We demonstrated that autoantibodies against B23 occur in ∼11% of sera obtained from patients with scleroderma. B23 seropositivity was related to pulmonary hypertension, antifibrillarin antibody, anti-RNP antibody, and decreased lung capacity. In multivariate analysis, B23 autoantibodies remained strongly associated with moderate-to-severe pulmonary hypertension and antifibrillarin antibodies. Conclusion. These data unite B23 with the group of nucleolar autoantigens targeted in scleroderma and thus focus attention on changes in the nucleolus that render its components immunogenic in this disease. The demonstration that antibodies to B23 are associated with an increased prevalence of pulmonary hypertension points to anti-B23 antibodies as a possible marker of a specific phenotype in scleroderma.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalArthritis Care and Research
Volume49
Issue number1
StatePublished - Feb 15 2003

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Pulmonary Hypertension
Autoantibodies
Antibodies
Anti-Idiotypic Antibodies
Lung Volume Measurements
Phenotype
Autoantigens
Serum
Multivariate Analysis
Enzyme-Linked Immunosorbent Assay
Demography
nucleophosmin

Keywords

  • Autoantibody
  • B23
  • Pulmonary hypertension
  • Scleroderma

ASJC Scopus subject areas

  • Rheumatology

Cite this

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title = "Autoantibodies against B23, a nucleolar phosphoprotein, occur in scleroderma and are associated with pulmonary hypertension",
abstract = "Objective. To determine whether the abundant nucleolar phosphoprotein B23 is a target of autoantibodies in scleroderma, and to examine the clinical phenotype associated with these antibodies. Methods. Ninety-two randomly selected scleroderma sera were screened by enzyme-linked immunosorbent assay against recombinant human B23. Demographic, clinical, and serologic parameters associated with B23 autoantibody status were examined. Results. We demonstrated that autoantibodies against B23 occur in ∼11{\%} of sera obtained from patients with scleroderma. B23 seropositivity was related to pulmonary hypertension, antifibrillarin antibody, anti-RNP antibody, and decreased lung capacity. In multivariate analysis, B23 autoantibodies remained strongly associated with moderate-to-severe pulmonary hypertension and antifibrillarin antibodies. Conclusion. These data unite B23 with the group of nucleolar autoantigens targeted in scleroderma and thus focus attention on changes in the nucleolus that render its components immunogenic in this disease. The demonstration that antibodies to B23 are associated with an increased prevalence of pulmonary hypertension points to anti-B23 antibodies as a possible marker of a specific phenotype in scleroderma.",
keywords = "Autoantibody, B23, Pulmonary hypertension, Scleroderma",
author = "Ulanet, {Danielle B.} and Fredrick Wigley and Allan Gelber and Antony Rosen",
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TY - JOUR

T1 - Autoantibodies against B23, a nucleolar phosphoprotein, occur in scleroderma and are associated with pulmonary hypertension

AU - Ulanet, Danielle B.

AU - Wigley, Fredrick

AU - Gelber, Allan

AU - Rosen, Antony

PY - 2003/2/15

Y1 - 2003/2/15

N2 - Objective. To determine whether the abundant nucleolar phosphoprotein B23 is a target of autoantibodies in scleroderma, and to examine the clinical phenotype associated with these antibodies. Methods. Ninety-two randomly selected scleroderma sera were screened by enzyme-linked immunosorbent assay against recombinant human B23. Demographic, clinical, and serologic parameters associated with B23 autoantibody status were examined. Results. We demonstrated that autoantibodies against B23 occur in ∼11% of sera obtained from patients with scleroderma. B23 seropositivity was related to pulmonary hypertension, antifibrillarin antibody, anti-RNP antibody, and decreased lung capacity. In multivariate analysis, B23 autoantibodies remained strongly associated with moderate-to-severe pulmonary hypertension and antifibrillarin antibodies. Conclusion. These data unite B23 with the group of nucleolar autoantigens targeted in scleroderma and thus focus attention on changes in the nucleolus that render its components immunogenic in this disease. The demonstration that antibodies to B23 are associated with an increased prevalence of pulmonary hypertension points to anti-B23 antibodies as a possible marker of a specific phenotype in scleroderma.

AB - Objective. To determine whether the abundant nucleolar phosphoprotein B23 is a target of autoantibodies in scleroderma, and to examine the clinical phenotype associated with these antibodies. Methods. Ninety-two randomly selected scleroderma sera were screened by enzyme-linked immunosorbent assay against recombinant human B23. Demographic, clinical, and serologic parameters associated with B23 autoantibody status were examined. Results. We demonstrated that autoantibodies against B23 occur in ∼11% of sera obtained from patients with scleroderma. B23 seropositivity was related to pulmonary hypertension, antifibrillarin antibody, anti-RNP antibody, and decreased lung capacity. In multivariate analysis, B23 autoantibodies remained strongly associated with moderate-to-severe pulmonary hypertension and antifibrillarin antibodies. Conclusion. These data unite B23 with the group of nucleolar autoantigens targeted in scleroderma and thus focus attention on changes in the nucleolus that render its components immunogenic in this disease. The demonstration that antibodies to B23 are associated with an increased prevalence of pulmonary hypertension points to anti-B23 antibodies as a possible marker of a specific phenotype in scleroderma.

KW - Autoantibody

KW - B23

KW - Pulmonary hypertension

KW - Scleroderma

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