Auger radiopharmaceutical therapy targeting prostate-specific membrane antigen

Ana P. Kiess, Il Minn, Ying Chen, Robert Hobbs, George Sgouros, Ronnie C. Mease, Mrudula Pullambhatla, Colette J. Shen, Catherine A. Foss, Martin G. Pomper

Research output: Contribution to journalArticle

Abstract

Auger electron emitters such as 125I have a high linear energy transfer and short range of emission (<10 μm), making them suitable for treating micrometastases while sparing normal tissues. We used a highly specific small molecule targeting the prostate-specific membrane antigen (PSMA) to deliver 125I to prostate cancer cells. Methods: The PSMA-targeting Auger emitter 2-[3-[1-carboxy-5-(4-125Iiodo- benzoylamino)-pentyl]-ureido]-pentanedioic acid (125I-DCIBzL) was synthesized. DNA damage (via phosphorylated H2A histone family member X staining) and clonogenic survival were tested in PSMA-positive (PSMA1) PC3 PIP and PSMA-negative (PSMA-) PC3 flu human prostate cancer cells after treatment with 125I-DCIBzL. Subcellular drug distribution was assessed with confocal microscopy using a related fluorescent PSMA-targeting compound YC-36. In vivo antitumor efficacy was tested in nude mice bearing PSMA1 PC3 PIP or PSMA- PC3 flu flank xenografts. Animals were administered (intravenously) 111 MBq (3 mCi) of 125I-DCIBzL, 111 MBq (3 mCi) of 125I-NaI, an equivalent amount of nonradiolabeled DCIBzL, or saline. Results: After treatment with 125I-DCIBzL, PSMA1 PC3 PIP cells exhibited increased DNA damage and decreased clonogenic survival when compared with PSMA- PC3 flu cells. Confocal microscopy of YC-36 showed drug distribution in the perinuclear area and plasma membrane. Animals bearing PSMA1 PC3 PIP tumors had significant tumor growth delay after treatment with 125I-DCIBzL, with only 1 mouse reaching 5 times the initial tumor volume by 60 d after treatment, compared with a median time to 5 times volume of less than 15 d for PSMA- PC3 flu tumors and all other treatment groups (P = 0.002 by log-rank test). Conclusion: PSMA-targeted radiopharmaceutical therapy with the Auger emitter 125I-DCIBzL yielded highly specific antitumor efficacy in vivo, suggesting promise for treatment of prostate cancer micrometastases.

Original languageEnglish (US)
Pages (from-to)1401-1407
Number of pages7
JournalJournal of Nuclear Medicine
Volume56
Issue number9
DOIs
StatePublished - Sep 1 2015

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Keywords

  • Auger
  • Iodine
  • PSMA
  • Prostate cancer
  • Radiopharmaceutical therapy
  • Radiotherapy

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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