Auer rods in the acute myeloid leukemias: frequency and methods of demonstration.

N. C. Jain, Christopher Cox, J. M. Bennett

Research output: Contribution to journalArticle

Abstract

One hundred and twenty-five cases of acute myeloid leukemia (AML) were reviewed for the presence of Auer rods by two peroxidase methods. The percentage of Auer rods recognized by Wright-Giemsa (WG) staining was 20.8% but three times higher by peroxidase staining techniques using either benzidine base as a substrate (PO method) or 3,3'-diaminobenzidine as a substrate (DAB method). Both PO and DAB methods were equally sensitive in identifying Auer rods, the higher percentage being in FAB types M2 and M4 and lower in M1, M3, and M5B, with none in M5A. Cell counts of at least 100 cells were as accurate as counting cells up to a total of 1000 consecutive blasts.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalHematological Oncology
Volume5
Issue number3
StatePublished - Jul 1987
Externally publishedYes

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Acute Myeloid Leukemia
Peroxidase
3,3'-Diaminobenzidine
Staining and Labeling
Cell Count

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Auer rods in the acute myeloid leukemias : frequency and methods of demonstration. / Jain, N. C.; Cox, Christopher; Bennett, J. M.

In: Hematological Oncology, Vol. 5, No. 3, 07.1987, p. 197-202.

Research output: Contribution to journalArticle

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