Attenuated pulmonary vascular response to acetylcholine in conscious dogs after cardiopulmonary bypass

P. Zanaboni, P. A. Murray, K. Fleisher, E. Tseng, P. P. Nyhan

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Abstract

We have demonstrated enhanced pulmonary vasoreactivity following cardiopulmonary bypass (CPB) in conscious dogs. To test the hypothesis that endothelial dysfunction mediates the enhanced vasoreactivity after cardiopulmonary bypass, we investigated the effect of acetylcholine on the pulmonary vascular pressure-flow (pulmonary arterial - left atrial pressure PAP-LAP/Q) relationship in chronically-instrumented conscious dogs (n=4) before and 3 days after CPB. PAP-LAP/Q relationships were generated by altering left pulmonary flow (OJ with a hydraulic occluder placed around the right pulmonary artery. After achieving matched levels of pulmonary vasoconstriction with infusion of U46619 both before and 3 days after closed-chest, hypothermie CPB (duration 2.5 hrs), the response to acetylcholine (0.01 to 10 mcg/kg/min) was determined. PAP-LAP (O=80 ml/ka/min) Pre-CPB Post-CPB ΔPAP-LAP ΔPAP-LAP U4 29.0+.7 26+1.2 Ach l 26.1+.9 -3+1.3 27.3+5.8 +1.3+5 Ach 10 19.2+1.8-10+1.2 25.6+3.4 -0.3+2.8 (p<0.01 vs U 4) These results indicate that the pulmonary vascular response to the endothelial-dependent vasodilator acetylcholine is attenuated following CPB. This endothelial dysfunction may be partly responsible for the enhanced pulmonary vascular reactivity which is observed post-CPB.

Original languageEnglish (US)
Pages (from-to)A106
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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