Attenuated glycogenolysis reduces glycolytic catabolite accumulation during ischemia in preconditioned rat hearts

Robert George Weiss, Cicero P. De Albuquerque, Koenraad Vandegaer, V. P. Chacko, Gary Gerstenblith

Research output: Contribution to journalArticle

Abstract

Prior transient episodes of ischemia ('ischemic preconditioning') reduce lactate accumulation and attenuate acidosis during a subsequent prolonged ischemic insult. The mechanisms responsible for attenuated glycolytic catabolite accumulation have not been established but may include earlier exhaustion of glycogen stores, slowed glycogenolysis before complete glycogen depletion, and/or inhibition of glycolysis. Simultaneous repeated measures of myocardial glycogen and the rates of glycolysis, glycogenolysis, glucose utilization, and glycolytic ATP production were obtained during total ischemia by 13C nuclear magnetic resonance spectroscopy in control and ischemia-preconditioned isolated rat hearts. Both [13C]glycolytic and [13C]glycogenolytic rates were significantly lower during total ischemia in preconditioned compared with control hearts (0.77±0.04 versus 1.06±0.06 μmol/min per gram wet weight [P13C]glycogen were still present. Importantly, the reduction in the rate of glycogenolysis was larger and out of proportion to the reduction in glycolysis and occurred despite an increase in glucose utilization in preconditioned hearts (2.23±0.15 versus 1.5±0.10 μmol/min per gram wet weight at 1.25 minutes, P

Original languageEnglish (US)
Pages (from-to)435-446
Number of pages12
JournalCirculation Research
Volume79
Issue number3
StatePublished - 1996

Fingerprint

Glycogenolysis
Glycogen
Glycolysis
Ischemia
Weights and Measures
Glucose
Ischemic Preconditioning
Acidosis
Lactic Acid
Magnetic Resonance Spectroscopy
Adenosine Triphosphate

Keywords

  • carbon-13 nuclear magnetic resonance
  • glycogen
  • glycolysis
  • lactate
  • phosphorylase

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Attenuated glycogenolysis reduces glycolytic catabolite accumulation during ischemia in preconditioned rat hearts. / Weiss, Robert George; De Albuquerque, Cicero P.; Vandegaer, Koenraad; Chacko, V. P.; Gerstenblith, Gary.

In: Circulation Research, Vol. 79, No. 3, 1996, p. 435-446.

Research output: Contribution to journalArticle

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AU - Gerstenblith, Gary

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