Attachment and biofilm formation of Mycobacterium marinum on a hydrophobic surface at the air interface

Mohammad R. Alavi, Hem D. Shukla, Brent Whitaker, Jill Arnold, Manoucher Shahamat

Research output: Contribution to journalArticle

Abstract

Properties of attachment of Mycobacterium marinum to hydrophobic surfaces and subsequent biofilm formation were investigated. Binding of M. marinum to polypropylene occured under aerobic and anaerobic/microaerophilic conditions. However, aerobic conditions were necessary for biofilms to persist. Highly non-polar organic solvents were found to efficiently remove attached bacteria from the polypropylene surface, indicating strong hydrophobic interactions between the M. marinum cell wall and the surface. Increased capsular material, occurring during stationary phase, correlated with a decrease in attachment of cells to polypropylene. A protein of approximately 40 kDa appears to be present in increased amounts during the stationary phase. The protein has been identified by LC MS/MS analysis as alanine dehydrogenase.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalWorld Journal of Microbiology and Biotechnology
Volume23
Issue number1
DOIs
StatePublished - Jan 1 2007

Keywords

  • Aerobic biofilm
  • Attachment
  • Culture condition
  • Hydrophobic interaction
  • M . marinum

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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