Atrial natriuretic factor receptors in rat kidney, adrenal gland, and brain: autoradiographic localization and fluid balance dependent changes.

D. R. Lynch, K. M. Braas, S. H. Snyder

Research output: Contribution to journalArticle

Abstract

Mammalian atria contain natriuretic peptides designated atrial natriuretic factors (ANF). Using in vitro autoradiography with 125I-labeled ANF, we have localized high-affinity (Kd = 150 pM) ANF binding sites to the glomeruli of the kidney, zona glomerulosa of the adrenal gland, and choroid plexus of the brain. The numbers of sites in both kidney and adrenal are increased in rats deprived of water; increases are detectable within 72 hr of water deprivation in the kidney and within 24 hr in the adrenal gland. Receptor numbers decline in rats given 2.0% NaCl as drinking water and in diabetic rats. The discrete localizations and dynamic alterations of these receptors suggest that ANF regulates fluid balance through diverse but coordinated effects on receptors in numerous organs including the kidney, adrenal, and brain.

Original languageEnglish (US)
Pages (from-to)3357-3361
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number10
StatePublished - May 1986
Externally publishedYes

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Atrial Natriuretic Factor Receptors
Water-Electrolyte Balance
Atrial Natriuretic Factor
Adrenal Glands
Kidney
Brain
Kidney Glomerulus
Zona Glomerulosa
Water Deprivation
Natriuretic Peptides
Choroid Plexus
Autoradiography
Drinking Water
Binding Sites
Water

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Mammalian atria contain natriuretic peptides designated atrial natriuretic factors (ANF). Using in vitro autoradiography with 125I-labeled ANF, we have localized high-affinity (Kd = 150 pM) ANF binding sites to the glomeruli of the kidney, zona glomerulosa of the adrenal gland, and choroid plexus of the brain. The numbers of sites in both kidney and adrenal are increased in rats deprived of water; increases are detectable within 72 hr of water deprivation in the kidney and within 24 hr in the adrenal gland. Receptor numbers decline in rats given 2.0{\%} NaCl as drinking water and in diabetic rats. The discrete localizations and dynamic alterations of these receptors suggest that ANF regulates fluid balance through diverse but coordinated effects on receptors in numerous organs including the kidney, adrenal, and brain.",
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