ATP synthesis, mitochondrial function, and steroid biosynthesis in rodent primary and tumor leydig cells

Andrew S. Midzak, Haolin Chen, Miguel A. Aon, Vassilios Papadopoulos, Barry R. Zirkin

Research output: Contribution to journalArticle

Abstract

Previous studies in MA-10 tumor Leydig cells demonstrated that disruption of the mitochondrial electron-transport chain (ETC), membrane potential (δψm), or ATP synthesis independently inhibited steroidogenesis. In contrast, studies of primary Leydig cells indicated that the ETC, δψm, and ATP synthesis cooperatively affected steroidogenesis. These results suggest significant differences between the two systems and call into question the extent to which results from tumor Leydig cells relate to primary cells. Thus, to further understand the similarities and differences between the two systems as well as the impact of ATP disruption on steroidogenesis, we performed comparative studies of MA-10 and primary Leydig cells under similar conditions of mitochondrial disruption. We show that mitochondrial ATP synthesis is critical for steroidogenesis in both primary and tumor Leydig cells. However, in striking contrast to primary cells, perturbation of δψm in MA-10 cells did not substantially decrease cellular ATP content, a perplexing finding because δψm powers the mitochondrial ATP synthase. Further studies revealed that a significant proportion of cellular ATP in MA-10 cells derives from glycolysis. In contrast, primary cells appear to be almost completely dependent on mitochondrial respiration for their energy provision. Inhibitor studies also suggested that the MA-10 ETC is impaired. This work underscores the importance of mitochondrial ATP for hormonestimulated steroid production in both MA-10 and primary Leydig cells while indicating that caution must be exercised in extrapolating data from tumor cells to primary tissue.

Original languageEnglish (US)
Pages (from-to)976-985
Number of pages10
JournalBiology of reproduction
Volume84
Issue number5
DOIs
StatePublished - May 1 2011

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Keywords

  • ATP
  • Glycolysis
  • Leydig cells
  • Mitochondria
  • Respiration
  • Steroid hormones/steroid hormone receptors
  • Testosterone

ASJC Scopus subject areas

  • Cell Biology

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