ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

Pejmon Pashai; Eric W. Kostuk; Luis E. Pilchard; Machiko Shirahata

doi:10.1007/978-94-007-4584-1-38

ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

Pejmon Pashai, Eric W. Kostuk, Luis E. Pilchard, Machiko Shirahata

Bloomberg School of Public Health

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

Original language	English (US)
Title of host publication	Arterial Chemoreception
Subtitle of host publication	From Molecules to Systems
Editors	Colin A. Nurse, Chris Peers, Constancio Gonzalez, Nanduri R. Prabhakar
Pages	279-285
Number of pages	7
DOIs	https://doi.org/10.1007/978-94-007-4584-1-38
State	Published - 2012

Publication series

Name	Advances in Experimental Medicine and Biology
Volume	758
ISSN (Print)	0065-2598

Keywords

Carotid body
Hyperoxia
Hypoxia
Neurotransmitter

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/978-94-007-4584-1-38

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Cite this

Pashai, P., Kostuk, E. W., Pilchard, L. E., & Shirahata, M. (2012). ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. In C. A. Nurse, C. Peers, C. Gonzalez, & N. R. Prabhakar (Eds.), Arterial Chemoreception: From Molecules to Systems (pp. 279-285). (Advances in Experimental Medicine and Biology; Vol. 758). https://doi.org/10.1007/978-94-007-4584-1-38

ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. / Pashai, Pejmon; Kostuk, Eric W.; Pilchard, Luis E.; Shirahata, Machiko.

Arterial Chemoreception: From Molecules to Systems. ed. / Colin A. Nurse; Chris Peers; Constancio Gonzalez; Nanduri R. Prabhakar. 2012. p. 279-285 (Advances in Experimental Medicine and Biology; Vol. 758).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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