ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

Pejmon Pashai, Eric W. Kostuk, Luis E. Pilchard, Machiko Shirahata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages279-285
Number of pages7
Volume758
DOIs
StatePublished - 2012

Publication series

NameAdvances in Experimental Medicine and Biology
Volume758
ISSN (Print)00652598

Fingerprint

Carotid Body
Inbred Strains Mice
Adenosine Triphosphate
Hypercapnia
Superior Cervical Ganglion
Neurotransmitter Agents
Centrifuges
Sepharose
Freezing
Melting point
Hypoxia

Keywords

  • Carotid body
  • Hyperoxia
  • Hypoxia
  • Neurotransmitter

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pashai, P., Kostuk, E. W., Pilchard, L. E., & Shirahata, M. (2012). ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. In Advances in Experimental Medicine and Biology (Vol. 758, pp. 279-285). (Advances in Experimental Medicine and Biology; Vol. 758). https://doi.org/10.1007/978-94-007-4584-1-38

ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. / Pashai, Pejmon; Kostuk, Eric W.; Pilchard, Luis E.; Shirahata, Machiko.

Advances in Experimental Medicine and Biology. Vol. 758 2012. p. 279-285 (Advances in Experimental Medicine and Biology; Vol. 758).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pashai, P, Kostuk, EW, Pilchard, LE & Shirahata, M 2012, ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. in Advances in Experimental Medicine and Biology. vol. 758, Advances in Experimental Medicine and Biology, vol. 758, pp. 279-285. https://doi.org/10.1007/978-94-007-4584-1-38
Pashai P, Kostuk EW, Pilchard LE, Shirahata M. ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. In Advances in Experimental Medicine and Biology. Vol. 758. 2012. p. 279-285. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-94-007-4584-1-38
Pashai, Pejmon ; Kostuk, Eric W. ; Pilchard, Luis E. ; Shirahata, Machiko. / ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. Advances in Experimental Medicine and Biology. Vol. 758 2012. pp. 279-285 (Advances in Experimental Medicine and Biology).
@inproceedings{783c0045cbb34e5487174eaf663fb6f3,
title = "ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains",
abstract = "The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar{\circledR} Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.",
keywords = "Carotid body, Hyperoxia, Hypoxia, Neurotransmitter",
author = "Pejmon Pashai and Kostuk, {Eric W.} and Pilchard, {Luis E.} and Machiko Shirahata",
year = "2012",
doi = "10.1007/978-94-007-4584-1-38",
language = "English (US)",
isbn = "9789400745834",
volume = "758",
series = "Advances in Experimental Medicine and Biology",
pages = "279--285",
booktitle = "Advances in Experimental Medicine and Biology",

}

TY - GEN

T1 - ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

AU - Pashai, Pejmon

AU - Kostuk, Eric W.

AU - Pilchard, Luis E.

AU - Shirahata, Machiko

PY - 2012

Y1 - 2012

N2 - The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

AB - The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

KW - Carotid body

KW - Hyperoxia

KW - Hypoxia

KW - Neurotransmitter

UR - http://www.scopus.com/inward/record.url?scp=84870466697&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870466697&partnerID=8YFLogxK

U2 - 10.1007/978-94-007-4584-1-38

DO - 10.1007/978-94-007-4584-1-38

M3 - Conference contribution

C2 - 23080173

AN - SCOPUS:84870466697

SN - 9789400745834

VL - 758

T3 - Advances in Experimental Medicine and Biology

SP - 279

EP - 285

BT - Advances in Experimental Medicine and Biology

ER -