ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

Pejmon Pashai, Eric W. Kostuk, Luis E. Pilchard, Machiko Shirahata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

Original languageEnglish (US)
Title of host publicationArterial Chemoreception
Subtitle of host publicationFrom Molecules to Systems
PublisherSpringer Science and Business Media, LLC
Number of pages7
ISBN (Print)9789400745834
StatePublished - 2012

Publication series

NameAdvances in Experimental Medicine and Biology
ISSN (Print)0065-2598


  • Carotid body
  • Hyperoxia
  • Hypoxia
  • Neurotransmitter

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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