ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains

Pejmon Pashai, Eric W. Kostuk, Luis E. Pilchard, Machiko Shirahata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.

Original languageEnglish (US)
Title of host publicationArterial Chemoreception
Subtitle of host publicationFrom Molecules to Systems
EditorsColin A. Nurse, Chris Peers, Constancio Gonzalez, Nanduri R. Prabhakar
Pages279-285
Number of pages7
DOIs
StatePublished - 2012

Publication series

NameAdvances in Experimental Medicine and Biology
Volume758
ISSN (Print)0065-2598

Keywords

  • Carotid body
  • Hyperoxia
  • Hypoxia
  • Neurotransmitter

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Pashai, P., Kostuk, E. W., Pilchard, L. E., & Shirahata, M. (2012). ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. In C. A. Nurse, C. Peers, C. Gonzalez, & N. R. Prabhakar (Eds.), Arterial Chemoreception: From Molecules to Systems (pp. 279-285). (Advances in Experimental Medicine and Biology; Vol. 758). https://doi.org/10.1007/978-94-007-4584-1-38