Abstract
The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Pages | 279-285 |
| Number of pages | 7 |
| Volume | 758 |
| DOIs | |
| State | Published - 2012 |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 758 |
| ISSN (Print) | 00652598 |
Fingerprint
Keywords
- Carotid body
- Hyperoxia
- Hypoxia
- Neurotransmitter
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains. / Pashai, Pejmon; Kostuk, Eric W.; Pilchard, Luis E.; Shirahata, Machiko.
Advances in Experimental Medicine and Biology. Vol. 758 2012. p. 279-285 (Advances in Experimental Medicine and Biology; Vol. 758).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - ATP release from the carotid bodies of DBA/2J and A/J inbred mouse strains
AU - Pashai, Pejmon
AU - Kostuk, Eric W.
AU - Pilchard, Luis E.
AU - Shirahata, Machiko
PY - 2012
Y1 - 2012
N2 - The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.
AB - The purposes of this study were to: (1) establish an effective method to measure the release of ATP from the mouse carotid body (CB) and (2) determine the release of ATP from the CB of the DBA/2 J (high hypoxic responder) and A/J (low hypoxic responder) mouse in response to hypoxia and hypercapnia. An incubation chamber was constructed utilizing a Costar® Spin-X Centrifuge Tube Filter. The filter was coated with low melting point agarose to hold 4 CBs or 4 superior cervical ganglia (SCG). Hypoxia did not increase ATP release from the CB of either strain. ATP increased in response to a normoxic/hypercapnic challenge in the DBA/2 J's CB but not in the A/J's CB. ATP release from the SCG was affected by neither hypoxia nor hypercapnia in both strains. Thus, we have concluded: (1) we successfully established a chamber system to measure ATP released from the mouse CB; (2) ATP may not be an excitatory neurotransmitter in the CB of these mice under hypoxia; (3) ATP may be a neurotransmitter in the CB of the DBA/2 J mouse strain during hypercapnia.
KW - Carotid body
KW - Hyperoxia
KW - Hypoxia
KW - Neurotransmitter
UR - http://www.scopus.com/inward/record.url?scp=84870466697&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870466697&partnerID=8YFLogxK
U2 - 10.1007/978-94-007-4584-1-38
DO - 10.1007/978-94-007-4584-1-38
M3 - Conference contribution
C2 - 23080173
AN - SCOPUS:84870466697
SN - 9789400745834
VL - 758
T3 - Advances in Experimental Medicine and Biology
SP - 279
EP - 285
BT - Advances in Experimental Medicine and Biology
ER -