TY - CHAP
T1 - ATP and GTP hydrolysis assays (TLC)
AU - Rajagopal, Vaishnavi
AU - Lorsch, Jon R.
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Many biochemical reactions that occur within the cell are thermodynamically unfavorable. However, when these reactions are coupled to NTP (nucleoside triphosphate) hydrolysis, the energy derived from the hydrolysis of the phosphodiester bond helps drive the reaction in the favorable direction. Examples of such proteins can be found in almost all facets of cellular metabolism: glycolysis and the TCA cycle, protein biosynthesis, DNA and RNA metabolism, cellular trafficking, cell signaling, cell division etc. Thus, characterization of the NTPase activity of these proteins in vitro can help in understanding the role of the protein in complex cellular processes.
AB - Many biochemical reactions that occur within the cell are thermodynamically unfavorable. However, when these reactions are coupled to NTP (nucleoside triphosphate) hydrolysis, the energy derived from the hydrolysis of the phosphodiester bond helps drive the reaction in the favorable direction. Examples of such proteins can be found in almost all facets of cellular metabolism: glycolysis and the TCA cycle, protein biosynthesis, DNA and RNA metabolism, cellular trafficking, cell signaling, cell division etc. Thus, characterization of the NTPase activity of these proteins in vitro can help in understanding the role of the protein in complex cellular processes.
KW - Adenosine triphosphate (ATP) hydrolysis
KW - Guanosine triphosphate (ATP) hydrolysis
KW - Nucleoside diphosphate (NDP)
KW - Nucleoside triphosphate (NTP)
KW - Preparation of the thin-layer chromatography plate and chamber
KW - Thin-layer chromatography (TLC) separation
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U2 - 10.1016/B978-0-12-420067-8.00025-8
DO - 10.1016/B978-0-12-420067-8.00025-8
M3 - Chapter
C2 - 24182937
AN - SCOPUS:84887246262
SN - 9780124200678
T3 - Methods in Enzymology
SP - 325
EP - 334
BT - Laboratory Methods in Enzymology
PB - Academic Press Inc.
ER -