ATP and GTP hydrolysis assays (TLC)

Vaishnavi Rajagopal, Jon R. Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations


Many biochemical reactions that occur within the cell are thermodynamically unfavorable. However, when these reactions are coupled to NTP (nucleoside triphosphate) hydrolysis, the energy derived from the hydrolysis of the phosphodiester bond helps drive the reaction in the favorable direction. Examples of such proteins can be found in almost all facets of cellular metabolism: glycolysis and the TCA cycle, protein biosynthesis, DNA and RNA metabolism, cellular trafficking, cell signaling, cell division etc. Thus, characterization of the NTPase activity of these proteins in vitro can help in understanding the role of the protein in complex cellular processes.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationCell, Lipid and Carbohydrate
PublisherAcademic Press Inc.
Number of pages10
ISBN (Print)9780124200678
StatePublished - Jan 1 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Adenosine triphosphate (ATP) hydrolysis
  • Guanosine triphosphate (ATP) hydrolysis
  • Nucleoside diphosphate (NDP)
  • Nucleoside triphosphate (NTP)
  • Preparation of the thin-layer chromatography plate and chamber
  • Thin-layer chromatography (TLC) separation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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