Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy

Maria Ilyas, Mario Mietzsch, Shweta Kailasan, Elina Väisänen, Mengxiao Luo, Paul Chipman, J. Kennon Smith, Justin Kurian, Duncan Sousa, Robert McKenna, Maria Söderlund-Venermo, Mavis Agbandje-McKenna

Research output: Contribution to journalArticle

Abstract

Bufavirus strain 1 (BuV1), a member of the Protoparvovirus genus of the Parvoviridae, was first isolated from fecal samples of children with acute diarrhea in Burkina Faso. Since this initial discovery, BuVs have been isolated in several countries, including Finland, the Netherlands, and Bhutan, in pediatric patients exhibiting similar symptoms. Towards their characterization, the structures of virus-like particles of BuV1, BuV2, and BuV3, the current known genotypes, have been determined by cryo-electron microscopy and image reconstruction to 2.84, 3.79, and 3.25 Å, respectively. The BuVs, 65–73% identical in amino acid sequence, conserve the major viral protein, VP2, structure and general capsid surface features of parvoviruses. These include a core β-barrel (βB-βI), α-helix A, and large surface loops inserted between these elements in VP2. The capsid contains depressions at the icosahedral 2-fold and around the 5-fold axes, and has three separated protrusions surrounding the 3-fold axes. Structure comparison among the BuVs and to available parvovirus structures revealed capsid surface variations and capsid 3-fold protrusions that depart from the single pinwheel arrangement of the animal protoparvoviruses. These structures provide a platform to begin the molecular characterization of these potentially pathogenic viruses.

Original languageEnglish (US)
Article number22
JournalViruses
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

Cryoelectron Microscopy
Capsid
Parvovirus
Parvoviridae
Bhutan
Burkina Faso
Computer-Assisted Image Processing
Viral Proteins
Finland
Virion
Netherlands
Amino Acid Sequence
Diarrhea
Genotype
Pediatrics
Viruses

Keywords

  • Bufavirus
  • Cryo-EM and image reconstruction
  • Parvoviruses
  • Single-stranded DNA virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Ilyas, M., Mietzsch, M., Kailasan, S., Väisänen, E., Luo, M., Chipman, P., ... Agbandje-McKenna, M. (2018). Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy. Viruses, 10(1), [22]. https://doi.org/10.3390/v10010022

Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy. / Ilyas, Maria; Mietzsch, Mario; Kailasan, Shweta; Väisänen, Elina; Luo, Mengxiao; Chipman, Paul; Smith, J. Kennon; Kurian, Justin; Sousa, Duncan; McKenna, Robert; Söderlund-Venermo, Maria; Agbandje-McKenna, Mavis.

In: Viruses, Vol. 10, No. 1, 22, 01.01.2018.

Research output: Contribution to journalArticle

Ilyas, M, Mietzsch, M, Kailasan, S, Väisänen, E, Luo, M, Chipman, P, Smith, JK, Kurian, J, Sousa, D, McKenna, R, Söderlund-Venermo, M & Agbandje-McKenna, M 2018, 'Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy', Viruses, vol. 10, no. 1, 22. https://doi.org/10.3390/v10010022
Ilyas M, Mietzsch M, Kailasan S, Väisänen E, Luo M, Chipman P et al. Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy. Viruses. 2018 Jan 1;10(1). 22. https://doi.org/10.3390/v10010022
Ilyas, Maria ; Mietzsch, Mario ; Kailasan, Shweta ; Väisänen, Elina ; Luo, Mengxiao ; Chipman, Paul ; Smith, J. Kennon ; Kurian, Justin ; Sousa, Duncan ; McKenna, Robert ; Söderlund-Venermo, Maria ; Agbandje-McKenna, Mavis. / Atomic resolution structures of human bufaviruses determined by cryo-electron microscopy. In: Viruses. 2018 ; Vol. 10, No. 1.
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