Atomic force microscopy and dissection of gap junctions

Jan H. Hoh, Ratneshwar Lal, Scott A. John, Jean Paul Revel, Morton F. Arnsdorf

Research output: Contribution to journalArticlepeer-review

Abstract

An atomic force microscope (AFM) was used to study the structure of isolated hepatic gap junctions in phosphate-buffered saline (PBS). The thickness of these gap junctions appears to be 14.4 nanometers, close to the dimensions reported by electron microscopy (EM). When an increasing force is applied to the microscope tip, the top membrane of the gap junction can be "dissected" away, leaving the extracellular domains of the bottom membrane exposed. When such "force dissection" is performed on samples both trypsinized and fixed with glutaraldehyde, the hexagonal array of gap junction hemichannels is revealed, with a center-to-center spacing of 9.1 nanometers.

Original languageEnglish (US)
Pages (from-to)1405-1408
Number of pages4
JournalScience
Volume253
Issue number5026
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • General

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