TY - JOUR
T1 - Atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI) on a quadrupole ion trap mass spectrometer
AU - Moyer, Susanne C.
AU - Marzilli, Lisa A.
AU - Woods, Amina S.
AU - Laiko, Victor V.
AU - Doroshenko, Vladimir M.
AU - Cotter, Robert J.
N1 - Funding Information:
Funding for this work was provided by a contract (DABT63-99-1-0006) to RJC from the Defense Advanced Research Project Agency (DARPA). Support for SCM was provided by an NSF-GOALI grant (CHE 9634238). LAM was supported by an NIH grant (GM 54882). ASW was funded by NIDA Intramural Research Program, NIDA, NIH. The authors wish to thank Professor Gary Glish of the University of North Carolina at Chapel Hill and Dr. John Callahan of the Naval Research Laboratory for providing Figs. 2 and 3 , respectively. We also thank Professor Philip Cole of the Johns Hopkins University School of Medicine for providing us with the modified and unmodified histone H3 peptides.
PY - 2003/3/15
Y1 - 2003/3/15
N2 - A new atmospheric pressure ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was recently introduced by Laiko et al. [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652]. This source has been coupled to an orthogonal time-of-flight mass spectrometer [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652] and to an ion trap mass spectrometer (ITMS) [Anal. Chem. 72 (2000) 5239; Atmospheric Pressure MALDI/Ion Trap Mass Spectrometry, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000; An Atmospheric Pressure MALDI Probe for Use with ESI Source Interfaces, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000; Atmospheric Pressure Matrix-Assisted Laser Desorption Ionization, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000]. Here, we present the current status of the work involving the development of an AP MALDI source for an ITMS. In addition, we present recent work from our own laboratory to demonstrate the utility of this novel configuration.
AB - A new atmospheric pressure ionization technique, atmospheric pressure matrix-assisted laser desorption/ionization (AP MALDI), was recently introduced by Laiko et al. [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652]. This source has been coupled to an orthogonal time-of-flight mass spectrometer [U.S. Patent 5,965,884; Abstracts of the 4th International Symposium on Mass Spectrometry in the Health and Life Sciences, San Francisco, CA, 25-29 August 1998; Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Proceedings of the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, 1999; Anal. Chem. 72 (2000) 652] and to an ion trap mass spectrometer (ITMS) [Anal. Chem. 72 (2000) 5239; Atmospheric Pressure MALDI/Ion Trap Mass Spectrometry, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000; An Atmospheric Pressure MALDI Probe for Use with ESI Source Interfaces, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000; Atmospheric Pressure Matrix-Assisted Laser Desorption Ionization, Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 2000]. Here, we present the current status of the work involving the development of an AP MALDI source for an ITMS. In addition, we present recent work from our own laboratory to demonstrate the utility of this novel configuration.
KW - AP MALDI
KW - Acetylation
KW - ITMS
KW - Oligosaccharides
KW - Phosphorylation
KW - Protein digests
KW - Proteins
KW - Sulfation
KW - Tandem mass spectrometry
KW - Time-of-flight
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U2 - 10.1016/S1387-3806(02)00972-7
DO - 10.1016/S1387-3806(02)00972-7
M3 - Article
AN - SCOPUS:0037445418
SN - 1387-3806
VL - 226
SP - 133
EP - 150
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
IS - 1
ER -