TY - JOUR
T1 - Asynchronous antigen expression in B lineage acute lymphoblastic leukemia
AU - Hurwitz, C. A.
AU - Loken, M. R.
AU - Graham, M. L.
AU - Karp, J. E.
AU - Borowitz, M. J.
AU - Pullen, D. J.
AU - Civin, C. I.
PY - 1988
Y1 - 1988
N2 - Cell surface phenotypes of 113 B lineage acute lymphocytic leukemia (ALL) cases, defined by the presence of HLA-DR and at least one B-cell-specific antigen (either CD19, CD20, or CD22), were compared with antigen-defined stages of normal B lymphocyte development. The cases were first evaluated for expression of HLA-DR, CD19, CD34, CD10, CD20, and CD22 by indirect one-color immunofluorescence. Pairwise comparisons of cell surface marker expression were performed for each leukemic sample: no correlations were observed for paired antigen expression on the leukemic samples using antigens expressed either early or late during normal B lymphoid development. Complete immunophenotypes of the cases were then compared with normal B-cell developmental stages. Sixteen different complete immunophenotypes were observed on the leukemias that were not found in normal marrow; at least 78% of the cases demonstrated such 'asynchronous' combinations of B lymphoid-associated differentiation antigens. Several samples were subsequently studied by two-color immunofluorescence, and the presence of doubly labeled cells with 'asynchronous' antigen combinations was confirmed. These results indicate that the majority of B lineage leukemias exhibit 'developmental asynchrony', as compared with normal marrow B cells. The data further suggest that ALL cases do not accurately represent cells arrested at the stage where the leukemogenic event occurred. Rather, ALL appears to be a disease in which there may be maturation of leukemic blasts; but this maturation is 'asynchronous' when compared with the normal developmental process.
AB - Cell surface phenotypes of 113 B lineage acute lymphocytic leukemia (ALL) cases, defined by the presence of HLA-DR and at least one B-cell-specific antigen (either CD19, CD20, or CD22), were compared with antigen-defined stages of normal B lymphocyte development. The cases were first evaluated for expression of HLA-DR, CD19, CD34, CD10, CD20, and CD22 by indirect one-color immunofluorescence. Pairwise comparisons of cell surface marker expression were performed for each leukemic sample: no correlations were observed for paired antigen expression on the leukemic samples using antigens expressed either early or late during normal B lymphoid development. Complete immunophenotypes of the cases were then compared with normal B-cell developmental stages. Sixteen different complete immunophenotypes were observed on the leukemias that were not found in normal marrow; at least 78% of the cases demonstrated such 'asynchronous' combinations of B lymphoid-associated differentiation antigens. Several samples were subsequently studied by two-color immunofluorescence, and the presence of doubly labeled cells with 'asynchronous' antigen combinations was confirmed. These results indicate that the majority of B lineage leukemias exhibit 'developmental asynchrony', as compared with normal marrow B cells. The data further suggest that ALL cases do not accurately represent cells arrested at the stage where the leukemogenic event occurred. Rather, ALL appears to be a disease in which there may be maturation of leukemic blasts; but this maturation is 'asynchronous' when compared with the normal developmental process.
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U2 - 10.1182/blood.v72.1.299.299
DO - 10.1182/blood.v72.1.299.299
M3 - Article
C2 - 3291983
AN - SCOPUS:0023749289
SN - 0006-4971
VL - 72
SP - 299
EP - 307
JO - Blood
JF - Blood
IS - 1
ER -