Asynchronous antigen expression in B lineage acute lymphoblastic leukemia

C. A. Hurwitz, M. R. Loken, M. L. Graham, Judith Karp, Michael J Borowitz, D. J. Pullen, C. I. Civin

Research output: Contribution to journalArticle

Abstract

Cell surface phenotypes of 113 B lineage acute lymphocytic leukemia (ALL) cases, defined by the presence of HLA-DR and at least one B-cell-specific antigen (either CD19, CD20, or CD22), were compared with antigen-defined stages of normal B lymphocyte development. The cases were first evaluated for expression of HLA-DR, CD19, CD34, CD10, CD20, and CD22 by indirect one-color immunofluorescence. Pairwise comparisons of cell surface marker expression were performed for each leukemic sample: no correlations were observed for paired antigen expression on the leukemic samples using antigens expressed either early or late during normal B lymphoid development. Complete immunophenotypes of the cases were then compared with normal B-cell developmental stages. Sixteen different complete immunophenotypes were observed on the leukemias that were not found in normal marrow; at least 78% of the cases demonstrated such 'asynchronous' combinations of B lymphoid-associated differentiation antigens. Several samples were subsequently studied by two-color immunofluorescence, and the presence of doubly labeled cells with 'asynchronous' antigen combinations was confirmed. These results indicate that the majority of B lineage leukemias exhibit 'developmental asynchrony', as compared with normal marrow B cells. The data further suggest that ALL cases do not accurately represent cells arrested at the stage where the leukemogenic event occurred. Rather, ALL appears to be a disease in which there may be maturation of leukemic blasts; but this maturation is 'asynchronous' when compared with the normal developmental process.

Original languageEnglish (US)
Pages (from-to)299-307
Number of pages9
JournalBlood
Volume72
Issue number1
StatePublished - 1988

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
B-Lymphocytes
Antigens
Cells
HLA-DR Antigens
Fluorescent Antibody Technique
Leukemia
CD19 Antigens
Color
Bone Marrow
Lymphocytes
Differentiation Antigens
Phenotype

ASJC Scopus subject areas

  • Hematology

Cite this

Hurwitz, C. A., Loken, M. R., Graham, M. L., Karp, J., Borowitz, M. J., Pullen, D. J., & Civin, C. I. (1988). Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. Blood, 72(1), 299-307.

Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. / Hurwitz, C. A.; Loken, M. R.; Graham, M. L.; Karp, Judith; Borowitz, Michael J; Pullen, D. J.; Civin, C. I.

In: Blood, Vol. 72, No. 1, 1988, p. 299-307.

Research output: Contribution to journalArticle

Hurwitz, CA, Loken, MR, Graham, ML, Karp, J, Borowitz, MJ, Pullen, DJ & Civin, CI 1988, 'Asynchronous antigen expression in B lineage acute lymphoblastic leukemia', Blood, vol. 72, no. 1, pp. 299-307.
Hurwitz CA, Loken MR, Graham ML, Karp J, Borowitz MJ, Pullen DJ et al. Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. Blood. 1988;72(1):299-307.
Hurwitz, C. A. ; Loken, M. R. ; Graham, M. L. ; Karp, Judith ; Borowitz, Michael J ; Pullen, D. J. ; Civin, C. I. / Asynchronous antigen expression in B lineage acute lymphoblastic leukemia. In: Blood. 1988 ; Vol. 72, No. 1. pp. 299-307.
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