Asymmetric signal transduction in polarized ileal Na+-absorbing cells

Carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-γ1 only to the brush border

Seema Khurana, Sawsan Kreydiyyeh, Anna Aronzon, W. Alexandra Hoogerwerf, Sue Goo Rhee, Mark Donowitz, Michael E. Cohen

Research output: Contribution to journalArticle

Abstract

In ileal Na+ absorptive cells, carbachol inhibits NaCl absorption and its component brush-border Na+/H+ exchanger, acting via basolateral membrane (BLM) receptors. This carbachol effect involves brush-border but not BLM protein kinase C. In the present work we describe another asymmetric aspect of signal transduction in these epithelial cells, this time involving phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC). Thirty seconds and 1 min after carbachol treatment, brush-border PIP2-specific PLC activity increased, returning to control levels by 2.5 min. Involvement of brush-border tyrosine kinase(s) in this effect was suggested by inhibition of the carbachol effect on NaCl absorption by the tyrosine kinase inhibitor genistein, added to the mucosal but not the serosal surface. Luminal genistein pretreatment also prevented the carbachol-induced increase in brush-border PLC activity. In contrast, carbachol exposure did not change the BLM PIP2-specific PLC activity. Western analysis and immunoprecipitation demonstrated that PLC-γ1 is present in the brush border and that carbachol increases the PLC-γ1 amount in the brush border. Both the brush border and BLM contain PLC-β3 and a small amount of PLC-δ1 but no PLC-β1, whereas BLM lacks detectable PLC-γ1. No change in PLC-β3 or PLC-δ1 amount in the brush border occurred with carbachol exposure. No change in tyrosine phosphorylation of brush-border PLC-γ1 occurred with carbachol treatment. The Ca2+ ionophore A23187 did not alter PIP2-specific PLC activity in either the brush border or the BLM. These studies demonstrate that carbachol but not Ca2+ ionophore effects on brush-border NaCl absorption are associated with increases in brush-border but not BLM PIP2-specific PLC activity and in the amount of brush-border PLC-γ1, and involve tyrosine phosphorylation. This asymmetric aspect of epithelial signal transduction, together with the previous demonstration of localization of high-sensitivity IP3 stores to the apical membrane area in intestinal epithelial cells, shows that different aspects of signal transduction occur at the apical and basolateral membranes in epithelia and requires studies in both domains to define mechanisms of intracellular signalling.

Original languageEnglish (US)
Pages (from-to)509-518
Number of pages10
JournalBiochemical Journal
Volume313
Issue number2
StatePublished - Jan 15 1996

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Signal transduction
Carbachol
Type C Phospholipases
Brushes
Microvilli
Signal Transduction
Membranes
Phosphorylation
Genistein
Ionophores
Protein-Tyrosine Kinases
Tyrosine
Epithelial Cells
Sodium-Hydrogen Antiporter
Level control
Calcimycin
Phosphatidylinositols

ASJC Scopus subject areas

  • Biochemistry

Cite this

Asymmetric signal transduction in polarized ileal Na+-absorbing cells : Carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-γ1 only to the brush border. / Khurana, Seema; Kreydiyyeh, Sawsan; Aronzon, Anna; Hoogerwerf, W. Alexandra; Rhee, Sue Goo; Donowitz, Mark; Cohen, Michael E.

In: Biochemical Journal, Vol. 313, No. 2, 15.01.1996, p. 509-518.

Research output: Contribution to journalArticle

Khurana, Seema ; Kreydiyyeh, Sawsan ; Aronzon, Anna ; Hoogerwerf, W. Alexandra ; Rhee, Sue Goo ; Donowitz, Mark ; Cohen, Michael E. / Asymmetric signal transduction in polarized ileal Na+-absorbing cells : Carbachol activates brush-border but not basolateral-membrane PIP2-PLC and translocates PLC-γ1 only to the brush border. In: Biochemical Journal. 1996 ; Vol. 313, No. 2. pp. 509-518.
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abstract = "In ileal Na+ absorptive cells, carbachol inhibits NaCl absorption and its component brush-border Na+/H+ exchanger, acting via basolateral membrane (BLM) receptors. This carbachol effect involves brush-border but not BLM protein kinase C. In the present work we describe another asymmetric aspect of signal transduction in these epithelial cells, this time involving phosphatidylinositol 4,5-bisphosphate (PIP2)-specific phospholipase C (PLC). Thirty seconds and 1 min after carbachol treatment, brush-border PIP2-specific PLC activity increased, returning to control levels by 2.5 min. Involvement of brush-border tyrosine kinase(s) in this effect was suggested by inhibition of the carbachol effect on NaCl absorption by the tyrosine kinase inhibitor genistein, added to the mucosal but not the serosal surface. Luminal genistein pretreatment also prevented the carbachol-induced increase in brush-border PLC activity. In contrast, carbachol exposure did not change the BLM PIP2-specific PLC activity. Western analysis and immunoprecipitation demonstrated that PLC-γ1 is present in the brush border and that carbachol increases the PLC-γ1 amount in the brush border. Both the brush border and BLM contain PLC-β3 and a small amount of PLC-δ1 but no PLC-β1, whereas BLM lacks detectable PLC-γ1. No change in PLC-β3 or PLC-δ1 amount in the brush border occurred with carbachol exposure. No change in tyrosine phosphorylation of brush-border PLC-γ1 occurred with carbachol treatment. The Ca2+ ionophore A23187 did not alter PIP2-specific PLC activity in either the brush border or the BLM. These studies demonstrate that carbachol but not Ca2+ ionophore effects on brush-border NaCl absorption are associated with increases in brush-border but not BLM PIP2-specific PLC activity and in the amount of brush-border PLC-γ1, and involve tyrosine phosphorylation. This asymmetric aspect of epithelial signal transduction, together with the previous demonstration of localization of high-sensitivity IP3 stores to the apical membrane area in intestinal epithelial cells, shows that different aspects of signal transduction occur at the apical and basolateral membranes in epithelia and requires studies in both domains to define mechanisms of intracellular signalling.",
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