Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement

Matthew Wooten, Jonathan Snedeker, Zehra F. Nizami, Xinxing Yang, Rajesh Ranjan, Elizabeth Urban, Jee Min Kim, Joseph Gall, Jie Xiao, Xin Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Many stem cells undergo asymmetric division to produce a self-renewing stem cell and a differentiating daughter cell. Here we show that, similarly to H3, histone H4 is inherited asymmetrically in Drosophila melanogaster male germline stem cells undergoing asymmetric division. In contrast, both H2A and H2B are inherited symmetrically. By combining super-resolution microscopy and chromatin fiber analyses with proximity ligation assays on intact nuclei, we find that old H3 is preferentially incorporated by the leading strand, whereas newly synthesized H3 is enriched on the lagging strand. Using a sequential nucleoside analog incorporation assay, we detect a high incidence of unidirectional replication fork movement in testes-derived chromatin and DNA fibers. Biased fork movement coupled with a strand preference in histone incorporation would explain how asymmetric old and new H3 and H4 are established during replication. These results suggest a role for DNA replication in patterning epigenetic information in asymmetrically dividing cells in multicellular organisms.

Original languageEnglish (US)
Pages (from-to)732-743
Number of pages12
JournalNature Structural and Molecular Biology
Volume26
Issue number8
DOIs
StatePublished - Aug 1 2019

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement'. Together they form a unique fingerprint.

Cite this