Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion

Brian R. Daniels, Edward M. Perkins, Terrence M. Dobrowsky, Sean X. Sun, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

To generate cellular diversity in developing organisms while simultaneously maintaining the developmental potential of the germline, germ cells must be able to preferentially endow germline daughter cells with a cytoplasmic portion containing specialized cell fate determinants not inherited by somatic cells. In Caenorhabditis elegans, germline inheritance of the protein PIE-1 is accomplished by fi rst asymmetrically localizing the protein to the germplasm before cleavage and subsequently degrading residual levels of the protein in the somatic cytoplasm after cleavage. Despite its critical involvement in cell fate determination, the enrichment of germline determinants remains poorly understood. Here, combining live-cell fl uorescence methods and kinetic modeling, we demonstrate that the enrichment process does not involve protein immobilization, intracellular compartmentalization, or localized protein degradation. Instead, our results support a heterogeneous reaction/diffusion model for PIE-1 enrichment in which the diffusion coeffi cient of PIE-1 is reversibly reduced in the posterior, resulting in a stable protein gradient across the zygote at steady state.

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalJournal of Cell Biology
Volume184
Issue number4
DOIs
StatePublished - Feb 23 2009

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Zygote
Caenorhabditis elegans
Proteins
Germ Cells
Immobilization
Proteolysis
Cytoplasm

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion. / Daniels, Brian R.; Perkins, Edward M.; Dobrowsky, Terrence M.; Sun, Sean X.; Wirtz, Denis.

In: Journal of Cell Biology, Vol. 184, No. 4, 23.02.2009, p. 473-479.

Research output: Contribution to journalArticle

Daniels, Brian R. ; Perkins, Edward M. ; Dobrowsky, Terrence M. ; Sun, Sean X. ; Wirtz, Denis. / Asymmetric enrichment of PIE-1 in the Caenorhabditis elegans zygote mediated by binary counterdiffusion. In: Journal of Cell Biology. 2009 ; Vol. 184, No. 4. pp. 473-479.
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