Association study for gene polymorphism of folic acid/homocysteine metabolic pathway and nonsyndromic cleft lip with or without cleft palate in Chinese populations

Ping Wang, Hong Wang, Yah huei Wu-chou, Xiao qian Ye, Shang zhi Huang, Bing Shi, Kung yee Liang, Wei hua Cao, Tao Wu, Terei H. Beaty

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To explore the association between 18 candidate genes encoding enzymes on the folate/homocysteine metabolism pathway and non-syndromic cleft lip with or without cleft palate (NSCL/P) in Chinese populations.

METHODS: A total of 806 NSCL/P trios were drawn by an international consortium, which conducted a genome-wide association study using a case-parent trio design to investigate genes affecting risks to NSCL/P. The transmission disequilibrium test (TDT) was used for deviation from Mendelian expectations for 257 SNPs in 18 folate/homocysteine metabolism-related genes. The interactions between markers in these gene and environmental risk factors were also tested using conditional Logistic regressions.

RESULTS: Although four SNPs (rs6428977, rs12060264, rs7730643 and rs4920037) showed nominal significant association with NSCL/P in the TDT on 806 NSCL/P trios (P<0.05), no significant evidence of linkage and association remained in all the SNPs after Bonferroni correction. Similar tests for interactions between genes and maternal smoking, environmental tobacco smoke, alcohol consumption and multi-vitamin supplementation during pregnancy did not attain statistical significance after correction for multiple comparisons.

CONCLUSION: Folate/homocysteine metabolism-related genes could not influence the risk of NSCL/P.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalBeijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
Volume45
Issue number3
StatePublished - Jun 18 2013

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ASJC Scopus subject areas

  • Medicine(all)

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