Association of vitamin D and antimicrobial peptide production during late-phase allergic responses in the lung

Research output: Contribution to journalArticle

Abstract

Background: Vitamin D may play important roles in regulating immune responses and in defence against infectious diseases by effects on both innate and adaptive immune responses. Little is known regarding activation of vitamin D within airway tissues and its relationship to inflammation and antimicrobial responses. Objective: The objective of this study was to investigate the activation of vitamin D within the airways and to define relationships between vitamin D metabolites and measures of inflammatory and antimicrobial responses assessed by bronchoalveolar lavage (BAL) during late-phase responses following allergen challenge of allergic subjects. Methods: Segmental allergen challenge was performed with saline and allergen in 16 adult allergic subjects. BAL was performed in both saline and allergen-challenged sites 20-24 h. after challenge. Following extraction from BAL fluids, levels of 25-hydroxy-vitamin D (25(OH)D) and 1,25-dihydroxy-vitamin D (1,25(OH) 2D) were assayed by specific radioimmunoassays. The cleavage product of cathelicidin, LL-37, was assayed by ELISA. Cellular constituents and albumin were measured. Results: Levels of vitamin D metabolites were increased in concentrated BAL fluids after allergen compared to saline challenge. Levels of 1,25(OH) 2D increased from largely undetectable to 2.5 pm (median; range: 1-29.5; P = 0.005) while 25(OH)D increased from 3.2 (0.8-6.2) to 6.2 (1.5-184.9) nm (P = 0.0006). Levels of LL-37 increased from 2.1 (1.4-4.1) to 14.5 (2.2-106.7) ng/mL BAL (P = 0.0005). Levels of LL-37, 1,25(OH) 2D, and 25(OH)D following allergen challenge were correlated with each other (P <0.0001), cellular changes, and levels of albumin (P <0.001). Conclusions and Clinical Relevance: Levels of vitamin D metabolites, particularly 1,25(OH) 2D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin. Normalization to albumin suggested plasma exudation as a mechanism for the increases. The findings support a role for vitamin D in allergic and innate immune responses in the lung.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalClinical and Experimental Allergy
Volume42
Issue number3
DOIs
StatePublished - Mar 2012

Fingerprint

Vitamin D
Allergens
Lung
Peptides
Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid
Innate Immunity
Albumins
Inflammation
Adaptive Immunity
Serum Albumin
Radioimmunoassay
Communicable Diseases
Enzyme-Linked Immunosorbent Assay

Keywords

  • Allergen challenge
  • Allergic response
  • Bronchoalveolar lavage
  • Cathelicidin
  • Human
  • Inflammation
  • LL-37
  • Lung
  • Vitamin D

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

@article{7c19ba529c464a43bbf9ede3c7139f5c,
title = "Association of vitamin D and antimicrobial peptide production during late-phase allergic responses in the lung",
abstract = "Background: Vitamin D may play important roles in regulating immune responses and in defence against infectious diseases by effects on both innate and adaptive immune responses. Little is known regarding activation of vitamin D within airway tissues and its relationship to inflammation and antimicrobial responses. Objective: The objective of this study was to investigate the activation of vitamin D within the airways and to define relationships between vitamin D metabolites and measures of inflammatory and antimicrobial responses assessed by bronchoalveolar lavage (BAL) during late-phase responses following allergen challenge of allergic subjects. Methods: Segmental allergen challenge was performed with saline and allergen in 16 adult allergic subjects. BAL was performed in both saline and allergen-challenged sites 20-24 h. after challenge. Following extraction from BAL fluids, levels of 25-hydroxy-vitamin D (25(OH)D) and 1,25-dihydroxy-vitamin D (1,25(OH) 2D) were assayed by specific radioimmunoassays. The cleavage product of cathelicidin, LL-37, was assayed by ELISA. Cellular constituents and albumin were measured. Results: Levels of vitamin D metabolites were increased in concentrated BAL fluids after allergen compared to saline challenge. Levels of 1,25(OH) 2D increased from largely undetectable to 2.5 pm (median; range: 1-29.5; P = 0.005) while 25(OH)D increased from 3.2 (0.8-6.2) to 6.2 (1.5-184.9) nm (P = 0.0006). Levels of LL-37 increased from 2.1 (1.4-4.1) to 14.5 (2.2-106.7) ng/mL BAL (P = 0.0005). Levels of LL-37, 1,25(OH) 2D, and 25(OH)D following allergen challenge were correlated with each other (P <0.0001), cellular changes, and levels of albumin (P <0.001). Conclusions and Clinical Relevance: Levels of vitamin D metabolites, particularly 1,25(OH) 2D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin. Normalization to albumin suggested plasma exudation as a mechanism for the increases. The findings support a role for vitamin D in allergic and innate immune responses in the lung.",
keywords = "Allergen challenge, Allergic response, Bronchoalveolar lavage, Cathelicidin, Human, Inflammation, LL-37, Lung, Vitamin D",
author = "Liu, {Mark Chang Hwa} and Huiqing Xiao and Brown, {A. J.} and Ritter, {C. S.} and Schroeder, {John Thomas}",
year = "2012",
month = "3",
doi = "10.1111/j.1365-2222.2011.03879.x",
language = "English (US)",
volume = "42",
pages = "383--391",
journal = "Clinical and Experimental Allergy",
issn = "0954-7894",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Association of vitamin D and antimicrobial peptide production during late-phase allergic responses in the lung

AU - Liu, Mark Chang Hwa

AU - Xiao, Huiqing

AU - Brown, A. J.

AU - Ritter, C. S.

AU - Schroeder, John Thomas

PY - 2012/3

Y1 - 2012/3

N2 - Background: Vitamin D may play important roles in regulating immune responses and in defence against infectious diseases by effects on both innate and adaptive immune responses. Little is known regarding activation of vitamin D within airway tissues and its relationship to inflammation and antimicrobial responses. Objective: The objective of this study was to investigate the activation of vitamin D within the airways and to define relationships between vitamin D metabolites and measures of inflammatory and antimicrobial responses assessed by bronchoalveolar lavage (BAL) during late-phase responses following allergen challenge of allergic subjects. Methods: Segmental allergen challenge was performed with saline and allergen in 16 adult allergic subjects. BAL was performed in both saline and allergen-challenged sites 20-24 h. after challenge. Following extraction from BAL fluids, levels of 25-hydroxy-vitamin D (25(OH)D) and 1,25-dihydroxy-vitamin D (1,25(OH) 2D) were assayed by specific radioimmunoassays. The cleavage product of cathelicidin, LL-37, was assayed by ELISA. Cellular constituents and albumin were measured. Results: Levels of vitamin D metabolites were increased in concentrated BAL fluids after allergen compared to saline challenge. Levels of 1,25(OH) 2D increased from largely undetectable to 2.5 pm (median; range: 1-29.5; P = 0.005) while 25(OH)D increased from 3.2 (0.8-6.2) to 6.2 (1.5-184.9) nm (P = 0.0006). Levels of LL-37 increased from 2.1 (1.4-4.1) to 14.5 (2.2-106.7) ng/mL BAL (P = 0.0005). Levels of LL-37, 1,25(OH) 2D, and 25(OH)D following allergen challenge were correlated with each other (P <0.0001), cellular changes, and levels of albumin (P <0.001). Conclusions and Clinical Relevance: Levels of vitamin D metabolites, particularly 1,25(OH) 2D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin. Normalization to albumin suggested plasma exudation as a mechanism for the increases. The findings support a role for vitamin D in allergic and innate immune responses in the lung.

AB - Background: Vitamin D may play important roles in regulating immune responses and in defence against infectious diseases by effects on both innate and adaptive immune responses. Little is known regarding activation of vitamin D within airway tissues and its relationship to inflammation and antimicrobial responses. Objective: The objective of this study was to investigate the activation of vitamin D within the airways and to define relationships between vitamin D metabolites and measures of inflammatory and antimicrobial responses assessed by bronchoalveolar lavage (BAL) during late-phase responses following allergen challenge of allergic subjects. Methods: Segmental allergen challenge was performed with saline and allergen in 16 adult allergic subjects. BAL was performed in both saline and allergen-challenged sites 20-24 h. after challenge. Following extraction from BAL fluids, levels of 25-hydroxy-vitamin D (25(OH)D) and 1,25-dihydroxy-vitamin D (1,25(OH) 2D) were assayed by specific radioimmunoassays. The cleavage product of cathelicidin, LL-37, was assayed by ELISA. Cellular constituents and albumin were measured. Results: Levels of vitamin D metabolites were increased in concentrated BAL fluids after allergen compared to saline challenge. Levels of 1,25(OH) 2D increased from largely undetectable to 2.5 pm (median; range: 1-29.5; P = 0.005) while 25(OH)D increased from 3.2 (0.8-6.2) to 6.2 (1.5-184.9) nm (P = 0.0006). Levels of LL-37 increased from 2.1 (1.4-4.1) to 14.5 (2.2-106.7) ng/mL BAL (P = 0.0005). Levels of LL-37, 1,25(OH) 2D, and 25(OH)D following allergen challenge were correlated with each other (P <0.0001), cellular changes, and levels of albumin (P <0.001). Conclusions and Clinical Relevance: Levels of vitamin D metabolites, particularly 1,25(OH) 2D, were low within the airways and increased after allergen challenge. The increases correlated with the magnitude of inflammation and increases in cathelicidin. Normalization to albumin suggested plasma exudation as a mechanism for the increases. The findings support a role for vitamin D in allergic and innate immune responses in the lung.

KW - Allergen challenge

KW - Allergic response

KW - Bronchoalveolar lavage

KW - Cathelicidin

KW - Human

KW - Inflammation

KW - LL-37

KW - Lung

KW - Vitamin D

UR - http://www.scopus.com/inward/record.url?scp=84857271039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857271039&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2222.2011.03879.x

DO - 10.1111/j.1365-2222.2011.03879.x

M3 - Article

C2 - 22092530

AN - SCOPUS:84857271039

VL - 42

SP - 383

EP - 391

JO - Clinical and Experimental Allergy

JF - Clinical and Experimental Allergy

SN - 0954-7894

IS - 3

ER -