Association of the "major histocompatibility complex subregion" I-J determinant with bioactive glycosylation-inhibiting factor

Tatsumi Nakano, Yun Cai Liu, Toshifumi Mikayama, Hiroshi Watarai, Masaru Taniguchi, Kimishige Ishizaka

Research output: Contribution to journalArticle

Abstract

Murine suppressor T-cell hybridoma cells (231F1) secrete not only bioactive glycosylation-inhibiting factor (GIF) but also an inactive peptide comparable to bioactive GIF peptide in its molecular size and reactivity with anti-GIF; the amino acid sequence of the inactive peptide is identical to that of the bioactive homologue. The inactive GIF peptide in culture supernatant of both the 231Fl cells and a stable transfectant of human GIF cDNA in the murine suppressor T hybridoma selectively bound to Affi-Gel 10, whereas bioactive GIF peptides from the same sources failed to bind to the gel. The inactive cytosolic human GIF from the stable transfectant and Escherichia coli-derived recombinant human GIF also had affinity for Affi-Gel 10. Both the bioactive murine GIF peptide from the suppressor T hybridoma and bioactive recombinant human GIF from the stable transfectant bound to the anti-I-Jb monoclonal antibody H6 coupled to Affi-Gel. However, bioactive hGIF produced by a stable transfectant of human GIF cDNA in BMT10 cells failed to be retained in H6-coupled Affi-Gel. These results indicate that the I-J specificity is determined by the cell source of the GIF peptide and that the I-J determinant recognized by monoclonal antibody H6 does not represent a part of the primary amino acid sequence of GIF. It appears that the epitope is generated by a posttranslational modification of the peptide.

Original languageEnglish (US)
Pages (from-to)9196-9200
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number20
StatePublished - Sep 26 1995
Externally publishedYes

Fingerprint

Major Histocompatibility Complex
Peptides
Hybridomas
Gels
I-J-antigen
immunoglobulin-binding factors
Amino Acid Sequence
Complementary DNA
Monoclonal Antibodies
Peptide T
Post Translational Protein Processing
Epitopes
Escherichia coli
T-Lymphocytes

Keywords

  • Macropbage migration-inhibitory factor
  • Posttranslational modification
  • Suppressor T-cell factor

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Association of the "major histocompatibility complex subregion" I-J determinant with bioactive glycosylation-inhibiting factor. / Nakano, Tatsumi; Liu, Yun Cai; Mikayama, Toshifumi; Watarai, Hiroshi; Taniguchi, Masaru; Ishizaka, Kimishige.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 20, 26.09.1995, p. 9196-9200.

Research output: Contribution to journalArticle

Nakano, Tatsumi ; Liu, Yun Cai ; Mikayama, Toshifumi ; Watarai, Hiroshi ; Taniguchi, Masaru ; Ishizaka, Kimishige. / Association of the "major histocompatibility complex subregion" I-J determinant with bioactive glycosylation-inhibiting factor. In: Proceedings of the National Academy of Sciences of the United States of America. 1995 ; Vol. 92, No. 20. pp. 9196-9200.
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