Association of randall plaque with collagen fibers and membrane vesicles

Saeed R. Khan, Douglas E. Rodriguez, Laurie B. Gower, Manoj Monga

Research output: Contribution to journalArticle

Abstract

Purpose: Idiopathic calcium oxalate kidney stones develop by calcium oxalate crystal deposition on Randall plaque. The mechanisms involved in Randall plaque formation are still unclear. We hypothesized that Randall plaque formation is similar to that of vascular calcification, involving components of extracellular matrix, including membrane bound vesicles and collagen fibers. To verify our hypothesis we critically examined renal papillary tissue from patients with stones. Materials and Methods: We performed 4 mm cold cup biopsy of renal papillae on 15 patients with idiopathic stones undergoing percutaneous nephrolithotomy. Tissue was immediately fixed and processed for analysis by various light and electron microscopic techniques. Results: Spherulitic calcium phosphate crystals, the hallmark of Randall plaque, were seen in all samples examined, including in interstitium and laminated basement membrane of tubular epithelium. Large crystalline deposits were composed of dark elongated strands mixed with spherulites. Strands showed banded patterns similar to collagen. Crystal deposits were surrounded by collagen fibers and membrane bound vesicles. Energy dispersive x-ray microanalysis and electron diffraction identified the crystals as hydroxyapatite. Few kidneys were examined and urinary data were not available on all patients. Conclusions: Results showed that crystals in Randall plaque are associated with collagen and membrane bound vesicles. Collagen fibers appeared calcified and vesicles contained crystals. Crystal deposition in renal papillae may have started with membrane vesicle induced nucleation and grown by the further addition of crystals at the periphery in a collagen framework.

Original languageEnglish (US)
Pages (from-to)1094-1100
Number of pages7
JournalJournal of Urology
Volume187
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Collagen
Membranes
Kidney
Calcium Oxalate
Electrons
Vascular Calcification
Percutaneous Nephrostomy
Kidney Calculi
Durapatite
Basement Membrane
Extracellular Matrix
Epithelium
X-Rays
Biopsy
Light

Keywords

  • calcium
  • collagen
  • kidney
  • kidney calculi
  • kidney medulla

ASJC Scopus subject areas

  • Urology

Cite this

Khan, S. R., Rodriguez, D. E., Gower, L. B., & Monga, M. (2012). Association of randall plaque with collagen fibers and membrane vesicles. Journal of Urology, 187(3), 1094-1100. https://doi.org/10.1016/j.juro.2011.10.125

Association of randall plaque with collagen fibers and membrane vesicles. / Khan, Saeed R.; Rodriguez, Douglas E.; Gower, Laurie B.; Monga, Manoj.

In: Journal of Urology, Vol. 187, No. 3, 03.2012, p. 1094-1100.

Research output: Contribution to journalArticle

Khan, SR, Rodriguez, DE, Gower, LB & Monga, M 2012, 'Association of randall plaque with collagen fibers and membrane vesicles', Journal of Urology, vol. 187, no. 3, pp. 1094-1100. https://doi.org/10.1016/j.juro.2011.10.125
Khan, Saeed R. ; Rodriguez, Douglas E. ; Gower, Laurie B. ; Monga, Manoj. / Association of randall plaque with collagen fibers and membrane vesicles. In: Journal of Urology. 2012 ; Vol. 187, No. 3. pp. 1094-1100.
@article{9e5836e80d6c42d38c910ba32adf0b41,
title = "Association of randall plaque with collagen fibers and membrane vesicles",
abstract = "Purpose: Idiopathic calcium oxalate kidney stones develop by calcium oxalate crystal deposition on Randall plaque. The mechanisms involved in Randall plaque formation are still unclear. We hypothesized that Randall plaque formation is similar to that of vascular calcification, involving components of extracellular matrix, including membrane bound vesicles and collagen fibers. To verify our hypothesis we critically examined renal papillary tissue from patients with stones. Materials and Methods: We performed 4 mm cold cup biopsy of renal papillae on 15 patients with idiopathic stones undergoing percutaneous nephrolithotomy. Tissue was immediately fixed and processed for analysis by various light and electron microscopic techniques. Results: Spherulitic calcium phosphate crystals, the hallmark of Randall plaque, were seen in all samples examined, including in interstitium and laminated basement membrane of tubular epithelium. Large crystalline deposits were composed of dark elongated strands mixed with spherulites. Strands showed banded patterns similar to collagen. Crystal deposits were surrounded by collagen fibers and membrane bound vesicles. Energy dispersive x-ray microanalysis and electron diffraction identified the crystals as hydroxyapatite. Few kidneys were examined and urinary data were not available on all patients. Conclusions: Results showed that crystals in Randall plaque are associated with collagen and membrane bound vesicles. Collagen fibers appeared calcified and vesicles contained crystals. Crystal deposition in renal papillae may have started with membrane vesicle induced nucleation and grown by the further addition of crystals at the periphery in a collagen framework.",
keywords = "calcium, collagen, kidney, kidney calculi, kidney medulla",
author = "Khan, {Saeed R.} and Rodriguez, {Douglas E.} and Gower, {Laurie B.} and Manoj Monga",
year = "2012",
month = "3",
doi = "10.1016/j.juro.2011.10.125",
language = "English (US)",
volume = "187",
pages = "1094--1100",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Association of randall plaque with collagen fibers and membrane vesicles

AU - Khan, Saeed R.

AU - Rodriguez, Douglas E.

AU - Gower, Laurie B.

AU - Monga, Manoj

PY - 2012/3

Y1 - 2012/3

N2 - Purpose: Idiopathic calcium oxalate kidney stones develop by calcium oxalate crystal deposition on Randall plaque. The mechanisms involved in Randall plaque formation are still unclear. We hypothesized that Randall plaque formation is similar to that of vascular calcification, involving components of extracellular matrix, including membrane bound vesicles and collagen fibers. To verify our hypothesis we critically examined renal papillary tissue from patients with stones. Materials and Methods: We performed 4 mm cold cup biopsy of renal papillae on 15 patients with idiopathic stones undergoing percutaneous nephrolithotomy. Tissue was immediately fixed and processed for analysis by various light and electron microscopic techniques. Results: Spherulitic calcium phosphate crystals, the hallmark of Randall plaque, were seen in all samples examined, including in interstitium and laminated basement membrane of tubular epithelium. Large crystalline deposits were composed of dark elongated strands mixed with spherulites. Strands showed banded patterns similar to collagen. Crystal deposits were surrounded by collagen fibers and membrane bound vesicles. Energy dispersive x-ray microanalysis and electron diffraction identified the crystals as hydroxyapatite. Few kidneys were examined and urinary data were not available on all patients. Conclusions: Results showed that crystals in Randall plaque are associated with collagen and membrane bound vesicles. Collagen fibers appeared calcified and vesicles contained crystals. Crystal deposition in renal papillae may have started with membrane vesicle induced nucleation and grown by the further addition of crystals at the periphery in a collagen framework.

AB - Purpose: Idiopathic calcium oxalate kidney stones develop by calcium oxalate crystal deposition on Randall plaque. The mechanisms involved in Randall plaque formation are still unclear. We hypothesized that Randall plaque formation is similar to that of vascular calcification, involving components of extracellular matrix, including membrane bound vesicles and collagen fibers. To verify our hypothesis we critically examined renal papillary tissue from patients with stones. Materials and Methods: We performed 4 mm cold cup biopsy of renal papillae on 15 patients with idiopathic stones undergoing percutaneous nephrolithotomy. Tissue was immediately fixed and processed for analysis by various light and electron microscopic techniques. Results: Spherulitic calcium phosphate crystals, the hallmark of Randall plaque, were seen in all samples examined, including in interstitium and laminated basement membrane of tubular epithelium. Large crystalline deposits were composed of dark elongated strands mixed with spherulites. Strands showed banded patterns similar to collagen. Crystal deposits were surrounded by collagen fibers and membrane bound vesicles. Energy dispersive x-ray microanalysis and electron diffraction identified the crystals as hydroxyapatite. Few kidneys were examined and urinary data were not available on all patients. Conclusions: Results showed that crystals in Randall plaque are associated with collagen and membrane bound vesicles. Collagen fibers appeared calcified and vesicles contained crystals. Crystal deposition in renal papillae may have started with membrane vesicle induced nucleation and grown by the further addition of crystals at the periphery in a collagen framework.

KW - calcium

KW - collagen

KW - kidney

KW - kidney calculi

KW - kidney medulla

UR - http://www.scopus.com/inward/record.url?scp=84857057099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857057099&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2011.10.125

DO - 10.1016/j.juro.2011.10.125

M3 - Article

C2 - 22266007

AN - SCOPUS:84857057099

VL - 187

SP - 1094

EP - 1100

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 3

ER -