TY - JOUR
T1 - Association of macroalbuminuria with oxidized LDL and TGF-β in type 2 diabetic patients
T2 - A case-control study
AU - Nakhjavani, Manouchehr
AU - Esteghamati, Alireza
AU - Khalilzadeh, Omid
AU - Asgarani, Firouzeh
AU - Mansournia, Nasrin
AU - Abbasi, Mehrshad
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/6
Y1 - 2010/6
N2 - The pathogenesis of diabetic nephropathy, mainly characterized by macroalbuminuria, is still poorly understood, but it is reported that transforming growth factor-β (TGF-β) plays a key role. In vitro evidence suggests that administration of oxidized LDL (ox-LDL) can lead to upregulation of TGF-β by human glomerular mesangial cells. This study aimed to evaluate the association between macroalbuminuria, ox-LDL, and TGF-β in diabetic patients. A total of 77 type 2 diabetic patients with macroalbuminuria (albumin excretion rate: AER ≥ 300 mg/24 h) and 66 patients with normoalbuminuria (AER ≤ 30 mg/ 24 h) were recruited. Fasting blood samples were obtained and serum levels of ox-LDL and TGF-β were determined. Ox-LDL and TGF-β were significantly higher in patients with macroalbuminuria than in those with normoalbuminuria (98.93 ± 3.99 vs. 72.45 ± 2.48 U/l; P<0.001 and 6.46 ± 0.74 vs. 2.49 ± 0.39 ng/ml; P<0.001, respectively). In patients with macroalbuminuria, there was a significant correlation between Ox-LDL and TGF-β (r = 0.376; P<0.01). AER was significantly correlated to ox-LDL (r = 0.302; P<0.05) and TGF-β (r = 0.306; P<0.05) in macroalbuminuric patients. This association remained significant after adjustment for potential confounders. Adjustment for TGF-β (ox- LDL), attenuated the association of ox-LDL (TGF-β) with AER. In conclusion, this study demonstrated the association of TGF-β and ox-LDL with albuminuria in macroalbuminuric type 2 diabetic patients, and suggested that this relationship is highly mediated through the correlation between TGF-β and ox-LDL.
AB - The pathogenesis of diabetic nephropathy, mainly characterized by macroalbuminuria, is still poorly understood, but it is reported that transforming growth factor-β (TGF-β) plays a key role. In vitro evidence suggests that administration of oxidized LDL (ox-LDL) can lead to upregulation of TGF-β by human glomerular mesangial cells. This study aimed to evaluate the association between macroalbuminuria, ox-LDL, and TGF-β in diabetic patients. A total of 77 type 2 diabetic patients with macroalbuminuria (albumin excretion rate: AER ≥ 300 mg/24 h) and 66 patients with normoalbuminuria (AER ≤ 30 mg/ 24 h) were recruited. Fasting blood samples were obtained and serum levels of ox-LDL and TGF-β were determined. Ox-LDL and TGF-β were significantly higher in patients with macroalbuminuria than in those with normoalbuminuria (98.93 ± 3.99 vs. 72.45 ± 2.48 U/l; P<0.001 and 6.46 ± 0.74 vs. 2.49 ± 0.39 ng/ml; P<0.001, respectively). In patients with macroalbuminuria, there was a significant correlation between Ox-LDL and TGF-β (r = 0.376; P<0.01). AER was significantly correlated to ox-LDL (r = 0.302; P<0.05) and TGF-β (r = 0.306; P<0.05) in macroalbuminuric patients. This association remained significant after adjustment for potential confounders. Adjustment for TGF-β (ox- LDL), attenuated the association of ox-LDL (TGF-β) with AER. In conclusion, this study demonstrated the association of TGF-β and ox-LDL with albuminuria in macroalbuminuric type 2 diabetic patients, and suggested that this relationship is highly mediated through the correlation between TGF-β and ox-LDL.
KW - Albuminuria
KW - Diabetes mellitus
KW - Diabetic nephropathy
KW - Ox-LDL
KW - TGF-β
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U2 - 10.1007/s11255-009-9643-9
DO - 10.1007/s11255-009-9643-9
M3 - Article
C2 - 19768565
AN - SCOPUS:77956181479
SN - 0301-1623
VL - 42
SP - 487
EP - 492
JO - International Urology and Nephrology
JF - International Urology and Nephrology
IS - 2
ER -