Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription

M. Dettenhofer, S. Cen, B. A. Carlson, L. Kleiman, X. F. Yu

Research output: Contribution to journalArticle

Abstract

The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from nonpermissive H9 cells displayed no significant differences in the amount, ratio, or integrity of their protein composition relative to an isogenic wild-type virion. The amounts of the virion-associated viral genomic RNA and tRNA(3)/(Lys) were additionally present at normal levels in vif mutant virions. We demonstrate that Vif associates with RNA in vitro as well as with viral genomic RNA in virus-infected cells. A functionally conserved lentivirus Vif motif was found in the double-stranded RNA binding domain of Xenopus laevis, Xlrbpa. The natural intravirion reverse transcriptase products were markedly reduced in vif mutant virions. Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in the initiation of reverse transcription with recombinant reverse transcriptase. These data point to a novel role for Vif in the regulation of efficient reverse transcription through modulation of the virion nucleic acid components.

Original languageEnglish (US)
Pages (from-to)8938-8945
Number of pages8
JournalJournal of Virology
Volume74
Issue number19
DOIs
StatePublished - 2000

Fingerprint

reverse transcription
Human immunodeficiency virus 1
virion
Virion
Reverse Transcription
HIV-1
RNA
mutants
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
Viral RNA
genomics
RNA, Transfer, Lys
vif Genes
Lentivirus
RNA Viruses
Xenopus laevis
double-stranded RNA
protein composition
virus replication

ASJC Scopus subject areas

  • Immunology

Cite this

Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription. / Dettenhofer, M.; Cen, S.; Carlson, B. A.; Kleiman, L.; Yu, X. F.

In: Journal of Virology, Vol. 74, No. 19, 2000, p. 8938-8945.

Research output: Contribution to journalArticle

Dettenhofer, M. ; Cen, S. ; Carlson, B. A. ; Kleiman, L. ; Yu, X. F. / Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription. In: Journal of Virology. 2000 ; Vol. 74, No. 19. pp. 8938-8945.
@article{4116f9bf3b7349379b32d51c2496f8d5,
title = "Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription",
abstract = "The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from nonpermissive H9 cells displayed no significant differences in the amount, ratio, or integrity of their protein composition relative to an isogenic wild-type virion. The amounts of the virion-associated viral genomic RNA and tRNA(3)/(Lys) were additionally present at normal levels in vif mutant virions. We demonstrate that Vif associates with RNA in vitro as well as with viral genomic RNA in virus-infected cells. A functionally conserved lentivirus Vif motif was found in the double-stranded RNA binding domain of Xenopus laevis, Xlrbpa. The natural intravirion reverse transcriptase products were markedly reduced in vif mutant virions. Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in the initiation of reverse transcription with recombinant reverse transcriptase. These data point to a novel role for Vif in the regulation of efficient reverse transcription through modulation of the virion nucleic acid components.",
author = "M. Dettenhofer and S. Cen and Carlson, {B. A.} and L. Kleiman and Yu, {X. F.}",
year = "2000",
doi = "10.1128/JVI.74.19.8938-8945.2000",
language = "English (US)",
volume = "74",
pages = "8938--8945",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "19",

}

TY - JOUR

T1 - Association of human immunodeficiency virus type 1 Vif with RNA and its role in reverse transcription

AU - Dettenhofer, M.

AU - Cen, S.

AU - Carlson, B. A.

AU - Kleiman, L.

AU - Yu, X. F.

PY - 2000

Y1 - 2000

N2 - The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from nonpermissive H9 cells displayed no significant differences in the amount, ratio, or integrity of their protein composition relative to an isogenic wild-type virion. The amounts of the virion-associated viral genomic RNA and tRNA(3)/(Lys) were additionally present at normal levels in vif mutant virions. We demonstrate that Vif associates with RNA in vitro as well as with viral genomic RNA in virus-infected cells. A functionally conserved lentivirus Vif motif was found in the double-stranded RNA binding domain of Xenopus laevis, Xlrbpa. The natural intravirion reverse transcriptase products were markedly reduced in vif mutant virions. Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in the initiation of reverse transcription with recombinant reverse transcriptase. These data point to a novel role for Vif in the regulation of efficient reverse transcription through modulation of the virion nucleic acid components.

AB - The vif gene of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication, although the functional target of Vif remains elusive. HIV-1 vif mutant virions derived from nonpermissive H9 cells displayed no significant differences in the amount, ratio, or integrity of their protein composition relative to an isogenic wild-type virion. The amounts of the virion-associated viral genomic RNA and tRNA(3)/(Lys) were additionally present at normal levels in vif mutant virions. We demonstrate that Vif associates with RNA in vitro as well as with viral genomic RNA in virus-infected cells. A functionally conserved lentivirus Vif motif was found in the double-stranded RNA binding domain of Xenopus laevis, Xlrbpa. The natural intravirion reverse transcriptase products were markedly reduced in vif mutant virions. Moreover, purified vif mutant genomic RNA-primer tRNA complexes displayed severe defects in the initiation of reverse transcription with recombinant reverse transcriptase. These data point to a novel role for Vif in the regulation of efficient reverse transcription through modulation of the virion nucleic acid components.

UR - http://www.scopus.com/inward/record.url?scp=0033798414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033798414&partnerID=8YFLogxK

U2 - 10.1128/JVI.74.19.8938-8945.2000

DO - 10.1128/JVI.74.19.8938-8945.2000

M3 - Article

C2 - 10982337

AN - SCOPUS:0033798414

VL - 74

SP - 8938

EP - 8945

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 19

ER -