Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA

Gary L. Gallia; Nune Darbinian; Anna Tretiakova; Sameer A. Ansari; Jay Rappaport; John Brady; Margaret J. Wortman; Edward M. Johnson; Kamel Khalili

doi:10.1073/pnas.96.20.11572

Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA

Gary L. Gallia, Nune Darbinian, Anna Tretiakova, Sameer A. Ansari, Jay Rappaport, John Brady, Margaret J. Wortman, Edward M. Johnson, Kamel Khalili

Research output: Contribution to journal › Article › peer-review

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Abstract

The interaction between two regulatory proteins plays a crucial role in the control of several biological events, including gene transcription. In this report, we demonstrate that the interaction between the cellular sequence-specific single-stranded DNA binding protein Purα and the HIV type 1 (HIV-1) Tat protein is mediated by specific ribonucleic acids. The region of Tat that is important for its interaction with Purα includes the region demonstrated to bind Tat's viral RNA target, TAR. A 10-nucleotide GC-rich consensus sequence identified in RNAs associated with Purα derived from human U-87MG cells plays an important role in the Purα:Tat interaction as examined by an in vitro reconstitution assay. Furthermore; expression of the Purα-associated RNA in these cells enhances transcriptional activation of the HIV-1 promoter by Tat and Purα.

Original language	English (US)
Pages (from-to)	11572-11577
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	96
Issue number	20
DOIs	https://doi.org/10.1073/pnas.96.20.11572
State	Published - Sep 28 1999
Externally published	Yes

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title = "Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA",

abstract = "The interaction between two regulatory proteins plays a crucial role in the control of several biological events, including gene transcription. In this report, we demonstrate that the interaction between the cellular sequence-specific single-stranded DNA binding protein Purα and the HIV type 1 (HIV-1) Tat protein is mediated by specific ribonucleic acids. The region of Tat that is important for its interaction with Purα includes the region demonstrated to bind Tat's viral RNA target, TAR. A 10-nucleotide GC-rich consensus sequence identified in RNAs associated with Purα derived from human U-87MG cells plays an important role in the Purα:Tat interaction as examined by an in vitro reconstitution assay. Furthermore; expression of the Purα-associated RNA in these cells enhances transcriptional activation of the HIV-1 promoter by Tat and Purα.",

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T1 - Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA

AU - Gallia, Gary L.

AU - Darbinian, Nune

AU - Tretiakova, Anna

AU - Ansari, Sameer A.

AU - Rappaport, Jay

AU - Brady, John

AU - Wortman, Margaret J.

AU - Johnson, Edward M.

AU - Khalili, Kamel

PY - 1999/9/28

Y1 - 1999/9/28

N2 - The interaction between two regulatory proteins plays a crucial role in the control of several biological events, including gene transcription. In this report, we demonstrate that the interaction between the cellular sequence-specific single-stranded DNA binding protein Purα and the HIV type 1 (HIV-1) Tat protein is mediated by specific ribonucleic acids. The region of Tat that is important for its interaction with Purα includes the region demonstrated to bind Tat's viral RNA target, TAR. A 10-nucleotide GC-rich consensus sequence identified in RNAs associated with Purα derived from human U-87MG cells plays an important role in the Purα:Tat interaction as examined by an in vitro reconstitution assay. Furthermore; expression of the Purα-associated RNA in these cells enhances transcriptional activation of the HIV-1 promoter by Tat and Purα.

AB - The interaction between two regulatory proteins plays a crucial role in the control of several biological events, including gene transcription. In this report, we demonstrate that the interaction between the cellular sequence-specific single-stranded DNA binding protein Purα and the HIV type 1 (HIV-1) Tat protein is mediated by specific ribonucleic acids. The region of Tat that is important for its interaction with Purα includes the region demonstrated to bind Tat's viral RNA target, TAR. A 10-nucleotide GC-rich consensus sequence identified in RNAs associated with Purα derived from human U-87MG cells plays an important role in the Purα:Tat interaction as examined by an in vitro reconstitution assay. Furthermore; expression of the Purα-associated RNA in these cells enhances transcriptional activation of the HIV-1 promoter by Tat and Purα.

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Association of HIV-1 Tat with the cellular protein, Purα, is mediated by RNA

Abstract

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