@article{5053da04386f45a888368b59b038a2cb,
title = "Association of diamine oxidase and ornithine decarboxylase with maturing cells in rapidly proliferating epithelium",
abstract = "Diamine oxidase and ornithine decarboxylase activities are shown to have a parallel distribution across rat small intestine mucosa; levels of both enzyme activities are sharply higher in mature cells in the villus tip region than in proliferating cells in the crypt areas. Histidine decarboxylase levels were not measurable in the same cell preparations and aromatic-L-amino-acid decarboxylase activity was distributed in an opposite pattern to diamine oxidase and ornithine decarboxylase. The results suggest that intestinal diamine oxidase could be involved with polyamine metabolism. The new findings for ornithine decarboxylase suggest an in vivo role for polyamines in non-proliferative cells; rat small intestinal mucosa may be an excellent model for investigating the function of polyamines in regenerating cells.",
author = "Baylin, {Stephen B.} and Stevens, {Shelley A.} and Shakir, {K. M.Mohamed}",
note = "Funding Information: diamineo xidasea ctivitiesc ouldbe detectedu ndert hesec onditionsin each mixing experiment. In intestinaml ucosaw, e have thus establishead g eographicaaln d cell maturationre lationshipb etweend iamineo xidasea nd siteso f putrescinsey n-thesis.T he questiono f whetherth is associatioinn volvesa critical role for di-amineo xidasein putrescinme etabolismm ustawait correlationbs etweena c-tual levelso f putrescinean d the higherp olyamineasn dl evelso f the enzyme activity.T he synthesios f putrescinfer om ornithinev ia ornithined ecarboxy-lase appearsto be the rate limiting step in polyamines ynthesis\[ 7\]T.h is step is tightly controlleda nd putrescinep laysa role throughi ts ability to inhibit ornithined ecarboxylas\e[2 4,2 5\]and to inducet he formationo f a protein inhibitoro f this enzyme\ [26\]In. rat intestinaml ucosad, iamineo xidaseis in positiont o providea dditionarl egulationo f putrescinlee vels. A most importanrta mificationo f the presenfti ndingsi s the association of intestinaol rnithined ecarboxylaswei th the maturen on-proliferatincge lls of the villus tip region.A n increasedc apacityfo r polyaminep roductionv ia putrescinefo rmationh asb eenl inkedin the past to the increasedle velso f DNA, RNA and proteins ynthesins ecessartyo supportr apidlyd ividingc ells. In systemss uchas regeneratinlgiv er,h oweveri,d entificationo f the specific cells containingth e high ornithined ecarboxylasaen d the stageo f maturation of such cells has not been identifiedA. lso, in some cell cultures ystems\[ 27\], increaseisn ornithined ecarboxylasaec tivityh avec orrelatedp oorlywith sti-mulationo f DNA synthesias nd cell proliferativaec tivity.I n theses amec ells high correlationw asfound betweenin creasedp olyamines ynthesias nd syn-thesiso f messengeRrN A \[27\] .S imilarly,w hile somei nvestigator\s[2 8--30\] have found inhibitorso f polyamines ynthesiisn vitro and in vivo to decrease cell proliferationa nd DNA synthesios thers\ [31,3 2\]h aves eenn o effecto n thesep arameters. Few studiesh avebeen directedin vivo at localizationo f ornithined e-carboxylasteo variousc ell types.I n one previouss tudyof rat duodenaml u-cosa,B all et al. \[33\f]o undc onsiderabloer nithined ecarboxylasaec tivitya sso. ciatedw ith both villus tip cells and crypt cells of the duodenumis olated with a planinga pparatusT.h esew orkersf elt, thereforet,h atthe enzyme could be associatewd ith non-proliferatincge llsbut concludedth atno gradient in activityw asp resenat crosst he mucosa\ [28\]O. ur resultsn ow raise the questiono f whethert he high levelso f ornithined ecarboxylasfoe undin rapidly regeneratintgis suesc ouldbe more involvedw ith the rapid maturation of recentlyf ormedc ellsr athert hanwith activelyp roliferatingo nes.P oly-aminef ormationm aythus be more importanitn the proteins ynthetica ctivi-ties requiredo f maturec ellsthan in the synthetica ctivitiess upportingp roli-ferativea ctivity.I nvestigationosf thesep ossibilitiems aybe particularlyre - wardingi n small intestinacl ells.L ikewise,t he specificc ellsr esponsiblfeo r the high activityo f ornithined ecarboxylasine tissuess uchas regeneratinlgiv er must be a focus of increaseda ttention. This researchw ass upportedin part by NIH Grants 5-R01-18404an d 1 P50 HL 19157-01S. .B.B. is supportedb y NIH ResearchC areerD evelop-ment Award 5-K04-CA-000-27a,n d K.M.M.S. by NIH FellowshipA M 07109. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1978",
month = jul,
day = "3",
doi = "10.1016/0304-4165(78)90200-3",
language = "English (US)",
volume = "541",
pages = "415--419",
journal = "BBA - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "3",
}