TY - JOUR
T1 - Association of bacteroides fragilis with childhood diarrhea
AU - San Joaquin, Venusto H.
AU - Griffis, Judy C.
AU - Lee, Christopher
AU - Sears, Cynthia L.
N1 - Funding Information:
The advice and invaluable help by Lyle L. Myers, Ph.D. are truly appreciated (VSJ). We thank A. Abdelhamid, M.D.; Jason Sig-mon; P. Sarvepalli, M.D.; Joseph Covington; and Pirtham Raj for technical assistance. This study was supported by the Oklahoma Center for Advancement of Science and Technology (VSJ), and by an Institutional Grant Award (to CLS) from the NIH Biomedical Research Support Grant (no. SO7 RR05378) awarded to the Johns Hopkins University School of Medicine.
PY - 1995
Y1 - 1995
N2 - Enterotoxigenic strains of Bacteriodes fragilis (ETBF) have recently been found to be associated with diarrheal illness in Apache and Bangladeshi children. This study was conducted to define the role of ETBF in diarrhea of children in an urban setting. Fecal specimens from 991 children with diarrhea and 581 asymptomatic age-matched controls were cultured for B. fragilis (BF). The isolates were tested for enterotoxin production using a human colonic epithelial cell line. BF was isolated from 318 (32.1% of the patients and 123 (21.2% of the controls (p < 0.001). In children < 1 year old which comprised about 50% of both patients and controls, the BF isolation rates were comparable (26.5% vs 25.7%; p = 0.812), contrasting with the significant difference in isolation rates for children < 1 year (37.6% vs 16.5%; p = < 0.001). Overall, ETBF were identified in 4.4% of patients and 3.1% of controls (p = 0.2). However, ETBF were significantly associated with diarrheal disease in children 1-5 years (5.4% vs 1.8%; p = 0.033) and 1-10 years (4.8% vs 1.5%; p = 0.021) in age. ETBF were isolated the year round and comprised 14.4% and 15% of the BF isolated from the patients and controls, respectively, suggesting that part of the indigenous BF are inherently enterotoxin producers. In this study, BF and ETBF were associated with diarrheal illness in children 1-10 years old.
AB - Enterotoxigenic strains of Bacteriodes fragilis (ETBF) have recently been found to be associated with diarrheal illness in Apache and Bangladeshi children. This study was conducted to define the role of ETBF in diarrhea of children in an urban setting. Fecal specimens from 991 children with diarrhea and 581 asymptomatic age-matched controls were cultured for B. fragilis (BF). The isolates were tested for enterotoxin production using a human colonic epithelial cell line. BF was isolated from 318 (32.1% of the patients and 123 (21.2% of the controls (p < 0.001). In children < 1 year old which comprised about 50% of both patients and controls, the BF isolation rates were comparable (26.5% vs 25.7%; p = 0.812), contrasting with the significant difference in isolation rates for children < 1 year (37.6% vs 16.5%; p = < 0.001). Overall, ETBF were identified in 4.4% of patients and 3.1% of controls (p = 0.2). However, ETBF were significantly associated with diarrheal disease in children 1-5 years (5.4% vs 1.8%; p = 0.033) and 1-10 years (4.8% vs 1.5%; p = 0.021) in age. ETBF were isolated the year round and comprised 14.4% and 15% of the BF isolated from the patients and controls, respectively, suggesting that part of the indigenous BF are inherently enterotoxin producers. In this study, BF and ETBF were associated with diarrheal illness in children 1-10 years old.
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U2 - 10.3109/00365549509019011
DO - 10.3109/00365549509019011
M3 - Article
C2 - 8539543
AN - SCOPUS:0029124702
SN - 0036-5548
VL - 27
SP - 211
EP - 215
JO - Scandinavian Journal of Infectious Diseases
JF - Scandinavian Journal of Infectious Diseases
IS - 3
ER -