Monocyte (MN) surface human leukocyte antigen DR was examined in 10 patients with pulmonary tuberculosis (TB) and 12 healthy individuals using OK11, a mouse monoclonal antibody to human DR, in a 51Cr-release cytotoxicity assay. Of freshly isolated MNs from TB patients, 39.1% ± 4.4% (mean ± SEM) were DR+, compared with 57.2% ± 5.7% in healthy subjects (P <0.02). After 24 hr in culture, a sharp rise was observed in the TB group, to 78.1% ± 11.6% (P <0.005), compared with 64.9% ± 5.1% in the control group. The TB patient group could be subdivided on the basis of tuberculin purified protein derivative-induced [3H]thymidine incorporation in peripheral blood mononuclear cells (PBMCs). A significantly smaller fraction of MNs from tuberculin nonresponder TB patients was DR+ (34.6% ± 6.0%) compared with healthy controls (59.4% ± 8.6%; P <0.05). In the nonresponder group, a greater fraction of PBMCs was identifiable as MNs by cytochemical techniques (51.2% ± 3.6% vs 38.0% ± 5.0% in the responder group; P <0.02). Cell mixing experiments demonstrated increased suppressor activity of DR- MNs.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Infectious Diseases|
|State||Published - 1984|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health