Association between total immunoglobulin e and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes

N. Acevedo, D. Mercado, Candelaria Vergara, J. Sánchez, M. W. Kennedy, S. Jiménez, A. M. Fernández, M. Gutiérrez, L. Puerta, L. Caraballo

Research output: Contribution to journalArticle

Abstract

The 13q33-34 region harbours a susceptibility locus to Ascaris lumbricoides, although the underlying genes are unknown. Immunoglobulin (Ig)E and IgG confer protective immunity and here we sought to investigate in an endemic population whether LIG4, TNFSF13B and IRS2 genes influence IgE and IgG levels against Ascaris and the ABA-1 allergen as a putative resistance marker. Mite-allergic asthmatic patients were analysed for potential relationships between Ascaris predisposition and allergy. One thousand and sixty-four subjects from Cartagena, Colombia, were included. Single nucleotide polymorphisms (SNPs) were genotyped using TaqMan assays. Antibody levels were measured by enzyme-linked immunosorbent assay. Linear and logistic regressions were used to model effects of genotypes on antibody levels. The GG genotype of LIG4 (rs1805388) was associated with higher IgE levels to Ascaris compared with other genotypes. TNFSF13B (rs10508198) was associated positively with IgG levels against Ascaris extract and IgE levels against ABA-1. In asthmatics, IRS2 (rs2289046) was associated with high total IgE levels. Associations held up after correction by population stratification using a set of 52 ancestry markers, age, sex and disease status. There was no association with asthma or mite sensitization. In a tropical population, LIG4 and TNFSF13B polymorphisms are associated with specific IgE and IgG to Ascaris, supporting previous linkage studies implicating the 13q33 region. Our results suggest that genes protecting against parasite infections can be different to those predisposing to asthma and atopy.

Original languageEnglish (US)
Pages (from-to)282-290
Number of pages9
JournalClinical and Experimental Immunology
Volume157
Issue number2
DOIs
StatePublished - Aug 2009
Externally publishedYes

Fingerprint

Ascaris lumbricoides
Ascaris
Immunoglobulin E
Antibody Formation
Immunoglobulins
Immune System
Immunoglobulin G
Infection
Genes
Mites
Genotype
Asthma
Population
Parasitic Diseases
Colombia
Antibodies
Allergens
Single Nucleotide Polymorphism
Immunity
Linear Models

Keywords

  • ABA-1
  • Ascaris lumbricoides
  • Genetic association
  • IgE
  • Mites

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Association between total immunoglobulin e and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes. / Acevedo, N.; Mercado, D.; Vergara, Candelaria; Sánchez, J.; Kennedy, M. W.; Jiménez, S.; Fernández, A. M.; Gutiérrez, M.; Puerta, L.; Caraballo, L.

In: Clinical and Experimental Immunology, Vol. 157, No. 2, 08.2009, p. 282-290.

Research output: Contribution to journalArticle

Acevedo, N. ; Mercado, D. ; Vergara, Candelaria ; Sánchez, J. ; Kennedy, M. W. ; Jiménez, S. ; Fernández, A. M. ; Gutiérrez, M. ; Puerta, L. ; Caraballo, L. / Association between total immunoglobulin e and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes. In: Clinical and Experimental Immunology. 2009 ; Vol. 157, No. 2. pp. 282-290.
@article{988410ac07c64c6a8831b345729bd902,
title = "Association between total immunoglobulin e and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes",
abstract = "The 13q33-34 region harbours a susceptibility locus to Ascaris lumbricoides, although the underlying genes are unknown. Immunoglobulin (Ig)E and IgG confer protective immunity and here we sought to investigate in an endemic population whether LIG4, TNFSF13B and IRS2 genes influence IgE and IgG levels against Ascaris and the ABA-1 allergen as a putative resistance marker. Mite-allergic asthmatic patients were analysed for potential relationships between Ascaris predisposition and allergy. One thousand and sixty-four subjects from Cartagena, Colombia, were included. Single nucleotide polymorphisms (SNPs) were genotyped using TaqMan assays. Antibody levels were measured by enzyme-linked immunosorbent assay. Linear and logistic regressions were used to model effects of genotypes on antibody levels. The GG genotype of LIG4 (rs1805388) was associated with higher IgE levels to Ascaris compared with other genotypes. TNFSF13B (rs10508198) was associated positively with IgG levels against Ascaris extract and IgE levels against ABA-1. In asthmatics, IRS2 (rs2289046) was associated with high total IgE levels. Associations held up after correction by population stratification using a set of 52 ancestry markers, age, sex and disease status. There was no association with asthma or mite sensitization. In a tropical population, LIG4 and TNFSF13B polymorphisms are associated with specific IgE and IgG to Ascaris, supporting previous linkage studies implicating the 13q33 region. Our results suggest that genes protecting against parasite infections can be different to those predisposing to asthma and atopy.",
keywords = "ABA-1, Ascaris lumbricoides, Genetic association, IgE, Mites",
author = "N. Acevedo and D. Mercado and Candelaria Vergara and J. S{\'a}nchez and Kennedy, {M. W.} and S. Jim{\'e}nez and Fern{\'a}ndez, {A. M.} and M. Guti{\'e}rrez and L. Puerta and L. Caraballo",
year = "2009",
month = "8",
doi = "10.1111/j.1365-2249.2009.03948.x",
language = "English (US)",
volume = "157",
pages = "282--290",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Association between total immunoglobulin e and antibody responses to naturally acquired Ascaris lumbricoides infection and polymorphisms of immune system-related LIG4, TNFSF13B and IRS2 genes

AU - Acevedo, N.

AU - Mercado, D.

AU - Vergara, Candelaria

AU - Sánchez, J.

AU - Kennedy, M. W.

AU - Jiménez, S.

AU - Fernández, A. M.

AU - Gutiérrez, M.

AU - Puerta, L.

AU - Caraballo, L.

PY - 2009/8

Y1 - 2009/8

N2 - The 13q33-34 region harbours a susceptibility locus to Ascaris lumbricoides, although the underlying genes are unknown. Immunoglobulin (Ig)E and IgG confer protective immunity and here we sought to investigate in an endemic population whether LIG4, TNFSF13B and IRS2 genes influence IgE and IgG levels against Ascaris and the ABA-1 allergen as a putative resistance marker. Mite-allergic asthmatic patients were analysed for potential relationships between Ascaris predisposition and allergy. One thousand and sixty-four subjects from Cartagena, Colombia, were included. Single nucleotide polymorphisms (SNPs) were genotyped using TaqMan assays. Antibody levels were measured by enzyme-linked immunosorbent assay. Linear and logistic regressions were used to model effects of genotypes on antibody levels. The GG genotype of LIG4 (rs1805388) was associated with higher IgE levels to Ascaris compared with other genotypes. TNFSF13B (rs10508198) was associated positively with IgG levels against Ascaris extract and IgE levels against ABA-1. In asthmatics, IRS2 (rs2289046) was associated with high total IgE levels. Associations held up after correction by population stratification using a set of 52 ancestry markers, age, sex and disease status. There was no association with asthma or mite sensitization. In a tropical population, LIG4 and TNFSF13B polymorphisms are associated with specific IgE and IgG to Ascaris, supporting previous linkage studies implicating the 13q33 region. Our results suggest that genes protecting against parasite infections can be different to those predisposing to asthma and atopy.

AB - The 13q33-34 region harbours a susceptibility locus to Ascaris lumbricoides, although the underlying genes are unknown. Immunoglobulin (Ig)E and IgG confer protective immunity and here we sought to investigate in an endemic population whether LIG4, TNFSF13B and IRS2 genes influence IgE and IgG levels against Ascaris and the ABA-1 allergen as a putative resistance marker. Mite-allergic asthmatic patients were analysed for potential relationships between Ascaris predisposition and allergy. One thousand and sixty-four subjects from Cartagena, Colombia, were included. Single nucleotide polymorphisms (SNPs) were genotyped using TaqMan assays. Antibody levels were measured by enzyme-linked immunosorbent assay. Linear and logistic regressions were used to model effects of genotypes on antibody levels. The GG genotype of LIG4 (rs1805388) was associated with higher IgE levels to Ascaris compared with other genotypes. TNFSF13B (rs10508198) was associated positively with IgG levels against Ascaris extract and IgE levels against ABA-1. In asthmatics, IRS2 (rs2289046) was associated with high total IgE levels. Associations held up after correction by population stratification using a set of 52 ancestry markers, age, sex and disease status. There was no association with asthma or mite sensitization. In a tropical population, LIG4 and TNFSF13B polymorphisms are associated with specific IgE and IgG to Ascaris, supporting previous linkage studies implicating the 13q33 region. Our results suggest that genes protecting against parasite infections can be different to those predisposing to asthma and atopy.

KW - ABA-1

KW - Ascaris lumbricoides

KW - Genetic association

KW - IgE

KW - Mites

UR - http://www.scopus.com/inward/record.url?scp=67650683700&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650683700&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2249.2009.03948.x

DO - 10.1111/j.1365-2249.2009.03948.x

M3 - Article

C2 - 19604268

AN - SCOPUS:67650683700

VL - 157

SP - 282

EP - 290

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -