Association between TNF-α and Entamoeba histolytica diarrhea

Kristine M. Peterson, Jianfen Shu, Priya Duggal, Rashidul Haque, Dinesh Mondal, William A. Petri

Research output: Contribution to journalArticlepeer-review

Abstract

An association between tumor necrosis factor α (TNF-α) and Entamoeba histolytica diarrhea was assessed in a cohort of 138 non-related Bangladeshi children who have been prospectively followed since 2001. Peripheral blood mononuclear cells (PBMCs) obtained at study entry were purified, cultured, and stimulated with soluble amebic antigen before cytokine measurement from supernatant. Higher levels of TNF-α were associated with increased risk of first (P = 0.01) and recurrent E. histolytica-related diarrheal episodes (P = 0.005). Children who developed E. histolytica diarrhea had significantly higher TNF-α protein levels than those who experienced asymptomatic E. histolytica infection (P value = 0.027) or no infection (P value = 0.017). Microarray studies performed using RNA isolated from acute and convalescent whole blood and colon biopsy samples revealed higher but non-significant TNF-α messenger RNA (mRNA) levels in subjects with acute E. histolytica diarrhea compared with convalescence. We conclude that there is an association between higher TNF-α production and E. histolytica diarrhea.

Original languageEnglish (US)
Pages (from-to)620-625
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number4
DOIs
StatePublished - Apr 2010

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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