Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia

Luisella Bocchio Chiavetto, Francesco Boin, Roberta Zanardini, Maurizio Popoli, Anna Michelato, Stefano Bignotti, Gian Battista Tura, Massimo Gennarelli

Research output: Contribution to journalArticle

Abstract

Background: Schizophrenia is one of the most severe psychiatric disorders, with a worldwide incidence of 1%. Several reports show abnormal cytokine levels in psychotic patients and indicate a possible role of the immune response system in the pathogenesis of schizophrenia. Increased concentrations of interleukin 10 (IL-10) have been found in plasma of schizophrenic patients, suggesting its potential role as a candidate gene for susceptibility to schizophrenia. IL-10 gene maps on chromosome 1 (q31-q32), a locus associated with genetic susceptibility to schizophrenia. Three functional haplotypes of the gene (GCC, ACC, ATA) have been described, derived from different combinations of three "single nucleotide polymorphisms" and directly related to the expression levels of the protein. Methods: We analyzed allele, genotype, and haplotype distributions in an association case-control study involving 106 schizophrenic patients and 143 unrelated healthy volunteers using polymerase chain reaction (PCR)-Single Strand Conformation Polymorphism and PCR Restriction Fragment Length Polymorphism methods. Results: Our results show a significant increase of GCC homozygotes (the high IL-10-producing haplotype) in schizophrenic patients compared to control subjects (χ2 = 13, p = .023; odds ratio = 3.03; 95% confidence interval, 1.274-7.355). Conclusions: These data could partly explain the abnormal secretion of IL-10 occurring in schizophrenic patients in response to infections or different stressors and suggest a potential role of IL-10 as a candidate gene for susceptibility to schizophrenia.

Original languageEnglish (US)
Pages (from-to)480-484
Number of pages5
JournalBiological Psychiatry
Volume51
Issue number6
DOIs
StatePublished - Mar 15 2002
Externally publishedYes

Fingerprint

Interleukin-10
Haplotypes
Schizophrenia
Genes
Polymerase Chain Reaction
Chromosomes, Human, Pair 1
Homozygote
Genetic Predisposition to Disease
Restriction Fragment Length Polymorphisms
Single Nucleotide Polymorphism
Psychiatry
Case-Control Studies
Immune System
Healthy Volunteers
Alleles
Odds Ratio
Genotype
Confidence Intervals
Cytokines
Incidence

Keywords

  • Association study
  • Gene
  • Interleukin-10
  • Polymorphism
  • Promoter region
  • Schizophrenia

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Chiavetto, L. B., Boin, F., Zanardini, R., Popoli, M., Michelato, A., Bignotti, S., ... Gennarelli, M. (2002). Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia. Biological Psychiatry, 51(6), 480-484. https://doi.org/10.1016/S0006-3223(01)01324-5

Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia. / Chiavetto, Luisella Bocchio; Boin, Francesco; Zanardini, Roberta; Popoli, Maurizio; Michelato, Anna; Bignotti, Stefano; Tura, Gian Battista; Gennarelli, Massimo.

In: Biological Psychiatry, Vol. 51, No. 6, 15.03.2002, p. 480-484.

Research output: Contribution to journalArticle

Chiavetto, LB, Boin, F, Zanardini, R, Popoli, M, Michelato, A, Bignotti, S, Tura, GB & Gennarelli, M 2002, 'Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia', Biological Psychiatry, vol. 51, no. 6, pp. 480-484. https://doi.org/10.1016/S0006-3223(01)01324-5
Chiavetto LB, Boin F, Zanardini R, Popoli M, Michelato A, Bignotti S et al. Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia. Biological Psychiatry. 2002 Mar 15;51(6):480-484. https://doi.org/10.1016/S0006-3223(01)01324-5
Chiavetto, Luisella Bocchio ; Boin, Francesco ; Zanardini, Roberta ; Popoli, Maurizio ; Michelato, Anna ; Bignotti, Stefano ; Tura, Gian Battista ; Gennarelli, Massimo. / Association between promoter polymorphic haplotypes of interleukin-10 gene and schizophrenia. In: Biological Psychiatry. 2002 ; Vol. 51, No. 6. pp. 480-484.
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AU - Michelato, Anna

AU - Bignotti, Stefano

AU - Tura, Gian Battista

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AB - Background: Schizophrenia is one of the most severe psychiatric disorders, with a worldwide incidence of 1%. Several reports show abnormal cytokine levels in psychotic patients and indicate a possible role of the immune response system in the pathogenesis of schizophrenia. Increased concentrations of interleukin 10 (IL-10) have been found in plasma of schizophrenic patients, suggesting its potential role as a candidate gene for susceptibility to schizophrenia. IL-10 gene maps on chromosome 1 (q31-q32), a locus associated with genetic susceptibility to schizophrenia. Three functional haplotypes of the gene (GCC, ACC, ATA) have been described, derived from different combinations of three "single nucleotide polymorphisms" and directly related to the expression levels of the protein. Methods: We analyzed allele, genotype, and haplotype distributions in an association case-control study involving 106 schizophrenic patients and 143 unrelated healthy volunteers using polymerase chain reaction (PCR)-Single Strand Conformation Polymorphism and PCR Restriction Fragment Length Polymorphism methods. Results: Our results show a significant increase of GCC homozygotes (the high IL-10-producing haplotype) in schizophrenic patients compared to control subjects (χ2 = 13, p = .023; odds ratio = 3.03; 95% confidence interval, 1.274-7.355). Conclusions: These data could partly explain the abnormal secretion of IL-10 occurring in schizophrenic patients in response to infections or different stressors and suggest a potential role of IL-10 as a candidate gene for susceptibility to schizophrenia.

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