Association between aflatoxin-albumin adduct levels and tortilla consumption in Guatemalan adults

María F. Kroker-Lobos, Christian S. Alvarez, Alvaro Rivera-Andrade, Joshua W. Smith, Patricia Egner, Olga Torres, Mariana Lazo-Elizondo, Neal D. Freedman, Eliseo Guallar, Barry I. Graubard, Katherine A. McGlynn, Manuel Ramírez-Zea, John Davis Groopman

Research output: Contribution to journalArticle

Abstract

Aflatoxin B1 (AFB1) is a known human hepatocarcinogen and a recent study reported elevated AFB1 levels, measured by serum albumin biomarkers, among Guatemalan adults. While AFB1 can contaminate a variety of foodstuffs, including maize, Guatemala's main dietary staple, the relationship of maize intake to serum AFB1-albumin adducts levels in Guatemala has not been previously examined. As a result, a cross-sectional study was conducted among 461 Guatemalan adults living in five geographically distinct departments of the country. Participants provided a serum sample and completed a semi-quantitative food frequency questionnaire and a sociodemographic questionnaire. Multiple linear regression analysis was used to estimate the least square means (LSQ) and 95% confidence intervals (95% CI) of log-transformed AFB1-albumin adducts by quintiles of maize consumption in crude and adjusted models. Additionally, analyses of tortilla consumption and levels of maize processing were conducted. The median maize intake was 344.3 g per day [Interquartile Range (IQR): 252.2, 500.8], and the median serum AFB1-albumin adduct level was 8.4 pg/mg albumin (IQR: 3.8, 22.3). In adjusted analyses, there was no association between overall maize consumption and serum AFB1-albumin levels. However, there was a statistically significant association between tortilla consumption and AFB1-albumin levels (ptrend = 0.01). The LSM of AFB1-albumin was higher in the highest quintile of tortilla consumption compared to the lowest quintile [LSM:9.03 95%CI: 7.03,11.70 vs 6.23, 95%CI: 4.95,8.17, respectively]. These findings indicate that tortilla may be an important source of AFB1 exposure in the Guatemalan population. Therefore, efforts to control or mitigate AFB1 levels in contaminated maize used for tortillas may reduce overall exposure in this population.

Original languageEnglish (US)
Pages (from-to)465-471
Number of pages7
JournalToxicology Reports
Volume6
DOIs
StatePublished - Jan 1 2019

Fingerprint

Aflatoxin B1
Zea mays
Albumins
Guatemala
Serum
aflatoxin-albumin adduct
Association reactions
Biomarkers
Least-Squares Analysis
Linear regression
Regression analysis
Serum Albumin
Population
Linear Models
Cross-Sectional Studies
Regression Analysis
Confidence Intervals

Keywords

  • Aflatoxins
  • Consumption
  • Guatemala
  • Maize
  • Tortilla

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Association between aflatoxin-albumin adduct levels and tortilla consumption in Guatemalan adults. / Kroker-Lobos, María F.; Alvarez, Christian S.; Rivera-Andrade, Alvaro; Smith, Joshua W.; Egner, Patricia; Torres, Olga; Lazo-Elizondo, Mariana; Freedman, Neal D.; Guallar, Eliseo; Graubard, Barry I.; McGlynn, Katherine A.; Ramírez-Zea, Manuel; Groopman, John Davis.

In: Toxicology Reports, Vol. 6, 01.01.2019, p. 465-471.

Research output: Contribution to journalArticle

Kroker-Lobos, MF, Alvarez, CS, Rivera-Andrade, A, Smith, JW, Egner, P, Torres, O, Lazo-Elizondo, M, Freedman, ND, Guallar, E, Graubard, BI, McGlynn, KA, Ramírez-Zea, M & Groopman, JD 2019, 'Association between aflatoxin-albumin adduct levels and tortilla consumption in Guatemalan adults', Toxicology Reports, vol. 6, pp. 465-471. https://doi.org/10.1016/j.toxrep.2019.05.009
Kroker-Lobos MF, Alvarez CS, Rivera-Andrade A, Smith JW, Egner P, Torres O et al. Association between aflatoxin-albumin adduct levels and tortilla consumption in Guatemalan adults. Toxicology Reports. 2019 Jan 1;6:465-471. https://doi.org/10.1016/j.toxrep.2019.05.009
Kroker-Lobos, María F. ; Alvarez, Christian S. ; Rivera-Andrade, Alvaro ; Smith, Joshua W. ; Egner, Patricia ; Torres, Olga ; Lazo-Elizondo, Mariana ; Freedman, Neal D. ; Guallar, Eliseo ; Graubard, Barry I. ; McGlynn, Katherine A. ; Ramírez-Zea, Manuel ; Groopman, John Davis. / Association between aflatoxin-albumin adduct levels and tortilla consumption in Guatemalan adults. In: Toxicology Reports. 2019 ; Vol. 6. pp. 465-471.
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abstract = "Aflatoxin B1 (AFB1) is a known human hepatocarcinogen and a recent study reported elevated AFB1 levels, measured by serum albumin biomarkers, among Guatemalan adults. While AFB1 can contaminate a variety of foodstuffs, including maize, Guatemala's main dietary staple, the relationship of maize intake to serum AFB1-albumin adducts levels in Guatemala has not been previously examined. As a result, a cross-sectional study was conducted among 461 Guatemalan adults living in five geographically distinct departments of the country. Participants provided a serum sample and completed a semi-quantitative food frequency questionnaire and a sociodemographic questionnaire. Multiple linear regression analysis was used to estimate the least square means (LSQ) and 95{\%} confidence intervals (95{\%} CI) of log-transformed AFB1-albumin adducts by quintiles of maize consumption in crude and adjusted models. Additionally, analyses of tortilla consumption and levels of maize processing were conducted. The median maize intake was 344.3 g per day [Interquartile Range (IQR): 252.2, 500.8], and the median serum AFB1-albumin adduct level was 8.4 pg/mg albumin (IQR: 3.8, 22.3). In adjusted analyses, there was no association between overall maize consumption and serum AFB1-albumin levels. However, there was a statistically significant association between tortilla consumption and AFB1-albumin levels (ptrend = 0.01). The LSM of AFB1-albumin was higher in the highest quintile of tortilla consumption compared to the lowest quintile [LSM:9.03 95{\%}CI: 7.03,11.70 vs 6.23, 95{\%}CI: 4.95,8.17, respectively]. These findings indicate that tortilla may be an important source of AFB1 exposure in the Guatemalan population. Therefore, efforts to control or mitigate AFB1 levels in contaminated maize used for tortillas may reduce overall exposure in this population.",
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