Assimilation of NAD+ precursors in Candida glabrata

Biao Ma, Shih Jung Pan, Margaret L. Zupancic, Brendan P Cormack

Research output: Contribution to journalArticle

Abstract

The yeast pathogen Candida glabrata is a nicotinamide adenine dinucleotide (NAD+) auxotroph and its growth depends on the environmental supply of vitamin precursors of NAD+. C. glabrata salvage pathways defined in this article allow NAD+ to be synthesized from three compounds - nicotinic acid (NA), nicotinamide (NAM) and nicotinamide riboside (NR). NA is salvaged through a functional Preiss-Handler pathway. NAM is first converted to NA by nicotinamidase and then salvaged by the Preiss-Handler pathway. Salvage of NR in C. glabrata occurs via two routes. The first, in which NR is phosphorylated by the NR kinase Nrk1, is independent of the Preiss-Handler pathway. The second is a novel pathway in which NR is degraded by the nucleosidases Pnp1 and Urh1, with a minor role for Meu1, and ultimately converted to NAD+ via the nicotinamidase Pnc1 and the Preiss-Handler pathway. Using C. glabrata mutants whose growth depends exclusively on the external NA or NR supply, we also show that C. glabrata utilizes NR and to a lesser extent NA as NAD+ sources during disseminated infection.

Original languageEnglish (US)
Pages (from-to)14-25
Number of pages12
JournalMolecular Microbiology
Volume66
Issue number1
DOIs
StatePublished - Oct 2007

Fingerprint

Candida glabrata
Niacin
NAD
Nicotinamidase
Niacinamide
N-Glycosyl Hydrolases
Growth
nicotinamide-beta-riboside
Yeasts
Infection

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Assimilation of NAD+ precursors in Candida glabrata. / Ma, Biao; Pan, Shih Jung; Zupancic, Margaret L.; Cormack, Brendan P.

In: Molecular Microbiology, Vol. 66, No. 1, 10.2007, p. 14-25.

Research output: Contribution to journalArticle

Ma, Biao ; Pan, Shih Jung ; Zupancic, Margaret L. ; Cormack, Brendan P. / Assimilation of NAD+ precursors in Candida glabrata. In: Molecular Microbiology. 2007 ; Vol. 66, No. 1. pp. 14-25.
@article{e7b43634a99b4ac8919a7dc77089b055,
title = "Assimilation of NAD+ precursors in Candida glabrata",
abstract = "The yeast pathogen Candida glabrata is a nicotinamide adenine dinucleotide (NAD+) auxotroph and its growth depends on the environmental supply of vitamin precursors of NAD+. C. glabrata salvage pathways defined in this article allow NAD+ to be synthesized from three compounds - nicotinic acid (NA), nicotinamide (NAM) and nicotinamide riboside (NR). NA is salvaged through a functional Preiss-Handler pathway. NAM is first converted to NA by nicotinamidase and then salvaged by the Preiss-Handler pathway. Salvage of NR in C. glabrata occurs via two routes. The first, in which NR is phosphorylated by the NR kinase Nrk1, is independent of the Preiss-Handler pathway. The second is a novel pathway in which NR is degraded by the nucleosidases Pnp1 and Urh1, with a minor role for Meu1, and ultimately converted to NAD+ via the nicotinamidase Pnc1 and the Preiss-Handler pathway. Using C. glabrata mutants whose growth depends exclusively on the external NA or NR supply, we also show that C. glabrata utilizes NR and to a lesser extent NA as NAD+ sources during disseminated infection.",
author = "Biao Ma and Pan, {Shih Jung} and Zupancic, {Margaret L.} and Cormack, {Brendan P}",
year = "2007",
month = "10",
doi = "10.1111/j.1365-2958.2007.05886.x",
language = "English (US)",
volume = "66",
pages = "14--25",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Assimilation of NAD+ precursors in Candida glabrata

AU - Ma, Biao

AU - Pan, Shih Jung

AU - Zupancic, Margaret L.

AU - Cormack, Brendan P

PY - 2007/10

Y1 - 2007/10

N2 - The yeast pathogen Candida glabrata is a nicotinamide adenine dinucleotide (NAD+) auxotroph and its growth depends on the environmental supply of vitamin precursors of NAD+. C. glabrata salvage pathways defined in this article allow NAD+ to be synthesized from three compounds - nicotinic acid (NA), nicotinamide (NAM) and nicotinamide riboside (NR). NA is salvaged through a functional Preiss-Handler pathway. NAM is first converted to NA by nicotinamidase and then salvaged by the Preiss-Handler pathway. Salvage of NR in C. glabrata occurs via two routes. The first, in which NR is phosphorylated by the NR kinase Nrk1, is independent of the Preiss-Handler pathway. The second is a novel pathway in which NR is degraded by the nucleosidases Pnp1 and Urh1, with a minor role for Meu1, and ultimately converted to NAD+ via the nicotinamidase Pnc1 and the Preiss-Handler pathway. Using C. glabrata mutants whose growth depends exclusively on the external NA or NR supply, we also show that C. glabrata utilizes NR and to a lesser extent NA as NAD+ sources during disseminated infection.

AB - The yeast pathogen Candida glabrata is a nicotinamide adenine dinucleotide (NAD+) auxotroph and its growth depends on the environmental supply of vitamin precursors of NAD+. C. glabrata salvage pathways defined in this article allow NAD+ to be synthesized from three compounds - nicotinic acid (NA), nicotinamide (NAM) and nicotinamide riboside (NR). NA is salvaged through a functional Preiss-Handler pathway. NAM is first converted to NA by nicotinamidase and then salvaged by the Preiss-Handler pathway. Salvage of NR in C. glabrata occurs via two routes. The first, in which NR is phosphorylated by the NR kinase Nrk1, is independent of the Preiss-Handler pathway. The second is a novel pathway in which NR is degraded by the nucleosidases Pnp1 and Urh1, with a minor role for Meu1, and ultimately converted to NAD+ via the nicotinamidase Pnc1 and the Preiss-Handler pathway. Using C. glabrata mutants whose growth depends exclusively on the external NA or NR supply, we also show that C. glabrata utilizes NR and to a lesser extent NA as NAD+ sources during disseminated infection.

UR - http://www.scopus.com/inward/record.url?scp=34548692312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548692312&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2958.2007.05886.x

DO - 10.1111/j.1365-2958.2007.05886.x

M3 - Article

C2 - 17725566

AN - SCOPUS:34548692312

VL - 66

SP - 14

EP - 25

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 1

ER -