Assignment of the human pancreatic regenerating (reg) gene to chromosome 2p12

Riccardo Perfetti, Anita L. Hawkins, Constance A. Griffin, Josephine M. Egan, Michael E. Zenilman, Alan R. Shuldiner

Research output: Contribution to journalArticlepeer-review

Abstract

A cDNA termed reg (for regenerating gene) has been isolated and characterized from a rat pancreatic library. Expression of reg is markedly increased in regenerating islets and decreased when insulin gene expression is inhibited. These findings have led to the hypothesis that reg may be involved in the expansion of β-cell mass during regeneration as well as in the maintenance of normal β-cell function. The human reg gene has a high degree of similarity to the rat reg gene. To determine the chromosomal location of the human reg gene, we analyzed two panels of mouse- or hamster-human hybrid cell lines containing a single human chromosome or several different human chromosomes. DNA extracts from these cell lines were analyzed for the presence of the human reg gene by polymerase chain reaction. In addition, human metaphase chromosomes were used for fluorescence in situ hybridization to further confirm the chromosomal assignment and to determine the subchromosomal localization. With these approaches, we show that the human reg gene is located on the short arm of chromosome 2 near the centromere at band 2p12.

Original languageEnglish (US)
Pages (from-to)305-307
Number of pages3
JournalGenomics
Volume20
Issue number2
DOIs
StatePublished - Mar 15 1994

ASJC Scopus subject areas

  • Genetics

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