Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias

Jennifer A. Pietenpol, Nickolas Papadopoulos, Yuko Sato, Kenneth W Kinzler, Bert Vogelstein, Cynthia Friedman, Barbara J. Trask, James M. Roberts, Kenneth W. Kinzler, Janet D. Rowley, Bert Vogelstein

Research output: Contribution to journalArticle

Abstract

The p27Kip1 (p27) gene encodes an inducible inhibitor of cyclin-dependent kinase activity. Using a murine p27 cDNA as probe, we obtained a human cDNA clone and subsequently used it to isolate a genomic clone of this gene. The coding region of the human p27 gene was contained in two exons. Both the amino acid sequence and intron-exon organization of p27 were similar to those previously found for the related cyclin-dependent kinase inhibitor p21Waf1 (p21). The p27 gene was localized to chromosome band 12p13 by a combination of somatic cell hybrid and fluorescence in situ hybridization analyses. The p27 gene product is thought to control the leukocyte cell cycle and the 12p13 chromosomal band is known to be deleted in leukemias, suggesting that the p27 gene may act as a tumor suppressor gene in leukemias. Although p27 was found to reside in the minimal region of chromosomal loss in hematological malignancies, no mutations of p27 were observed in leukemia samples. Haploinsufficiency of p27 may confer a growth advantage to leukemia cells.

Original languageEnglish (US)
Pages (from-to)1206-1210
Number of pages5
JournalCancer Research
Volume55
Issue number6
StatePublished - Mar 15 1995

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Leukemia
Genes
Cyclin-Dependent Kinases
Exons
Complementary DNA
Clone Cells
Haploinsufficiency
Hybrid Cells
Hematologic Neoplasms
Cell Cycle Checkpoints
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
Introns
Amino Acid Sequence
Leukocytes
Chromosomes
Mutation
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias. / Pietenpol, Jennifer A.; Papadopoulos, Nickolas; Sato, Yuko; Kinzler, Kenneth W; Vogelstein, Bert; Friedman, Cynthia; Trask, Barbara J.; Roberts, James M.; Kinzler, Kenneth W.; Rowley, Janet D.; Vogelstein, Bert.

In: Cancer Research, Vol. 55, No. 6, 15.03.1995, p. 1206-1210.

Research output: Contribution to journalArticle

Pietenpol, JA, Papadopoulos, N, Sato, Y, Kinzler, KW, Vogelstein, B, Friedman, C, Trask, BJ, Roberts, JM, Kinzler, KW, Rowley, JD & Vogelstein, B 1995, 'Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias', Cancer Research, vol. 55, no. 6, pp. 1206-1210.
Pietenpol, Jennifer A. ; Papadopoulos, Nickolas ; Sato, Yuko ; Kinzler, Kenneth W ; Vogelstein, Bert ; Friedman, Cynthia ; Trask, Barbara J. ; Roberts, James M. ; Kinzler, Kenneth W. ; Rowley, Janet D. ; Vogelstein, Bert. / Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias. In: Cancer Research. 1995 ; Vol. 55, No. 6. pp. 1206-1210.
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