Assessment of variability in the plasma 7k SomaScan proteomics assay

Julián Candia, Gulzar N. Daya, Toshiko Tanaka, Luigi Ferrucci, Keenan A. Walker

Research output: Contribution to journalArticlepeer-review


SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.

Original languageEnglish (US)
Article number17147
JournalScientific reports
Issue number1
StatePublished - Dec 2022
Externally publishedYes

ASJC Scopus subject areas

  • General


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