Assessment of variability in the plasma 7k SomaScan proteomics assay

Julián Candia; Gulzar N. Daya; Toshiko Tanaka; Luigi Ferrucci; Keenan A. Walker

doi:10.1038/s41598-022-22116-0

Assessment of variability in the plasma 7k SomaScan proteomics assay

Julián Candia, Gulzar N. Daya, Toshiko Tanaka, Luigi Ferrucci, Keenan A. Walker

Research output: Contribution to journal › Article › peer-review

Abstract

SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.

Original language	English (US)
Article number	17147
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-22116-0
State	Published - Dec 2022
Externally published	Yes

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title = "Assessment of variability in the plasma 7k SomaScan proteomics assay",

abstract = "SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.",

author = "Juli{\'a}n Candia and Daya, {Gulzar N.} and Toshiko Tanaka and Luigi Ferrucci and Walker, {Keenan A.}",

note = "Funding Information: This research was supported entirely by the Intramural Research Program of the National Institute on Aging, NIH. We thank Brian Sellers (NIH), Kathryn Jenko and Jennifer Yoon (SomaLogic, Inc.) for useful discussions. Publisher Copyright: {\textcopyright} 2022, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.",

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doi = "10.1038/s41598-022-22116-0",

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AU - Ferrucci, Luigi

AU - Walker, Keenan A.

N1 - Funding Information: This research was supported entirely by the Intramural Research Program of the National Institute on Aging, NIH. We thank Brian Sellers (NIH), Kathryn Jenko and Jennifer Yoon (SomaLogic, Inc.) for useful discussions. Publisher Copyright: © 2022, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

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Assessment of variability in the plasma 7k SomaScan proteomics assay

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