TY - JOUR
T1 - Assessment of the influence of irrelevant IgE on allergic sensitivity to two independent allergens
AU - Schellenberg, R. Robert
AU - Adkinson, N. Franklin
N1 - Funding Information:
From the Division of Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine. Supported by Grant Al 11936 from the National Institute of Health Publication No. 320 from the O’Neill Research Laboratories, Good Samaritan Hospital, 5601 Loch Raven Blvd., Baltimore, Md. 21239. Received for publication July 1 I, 1978. Accepted for publication Aug. 1 1, 1978. Reprint requests to: R. Robert Schellenberg, M.D., Department of Immunology, University of Manitoba, 795 McDermot Ave., Winnipeg, Manitoba R3EOW3, Canada. *Recipient of MRC of Canada Fellowship Award.
PY - 1979/1
Y1 - 1979/1
N2 - Serum samples from 31 patients sensitive to both ragweed and rye grass were quantitated for IgE specific for ragweed antigen E (AgE) and rye grass group I (rye I) antigens employing the previously described radioallergosorbent test (RAST) elution technique. IgE anti-AgE ranged from 0.4 to 320 ng/ml and comprised 0.4% to 14.2% of total serum IgE. The same sera contained IgE anti-rye I ranging from 7.9 to 1,168 ng/ml, comprising 1.6% to 29.6% of total serum IgE. A new theoretical parameter (RF), representing the percent of total IgE-Fc receptors of target cells occupied by allergen-specific IgE, was calculated for each IgE specificity by using the determinations of allergen-specific IgE antibody, total serum IgE, and assuming an equilibrium constant for binding of IgE molecules to basophils of 109M-1. This "% occupancy" parameter correlated with skin test titration, basophil cell sensitivity, and hay fever symptom scores as well as, but not better than, the absolute values of allergen-specific IgE. This finding suggests that in most atopic patients, the quantity of irrelevant IgE plays a relatively minor role in determining allergic sensitivity to a given allergen. The implications of this finding are discussed.
AB - Serum samples from 31 patients sensitive to both ragweed and rye grass were quantitated for IgE specific for ragweed antigen E (AgE) and rye grass group I (rye I) antigens employing the previously described radioallergosorbent test (RAST) elution technique. IgE anti-AgE ranged from 0.4 to 320 ng/ml and comprised 0.4% to 14.2% of total serum IgE. The same sera contained IgE anti-rye I ranging from 7.9 to 1,168 ng/ml, comprising 1.6% to 29.6% of total serum IgE. A new theoretical parameter (RF), representing the percent of total IgE-Fc receptors of target cells occupied by allergen-specific IgE, was calculated for each IgE specificity by using the determinations of allergen-specific IgE antibody, total serum IgE, and assuming an equilibrium constant for binding of IgE molecules to basophils of 109M-1. This "% occupancy" parameter correlated with skin test titration, basophil cell sensitivity, and hay fever symptom scores as well as, but not better than, the absolute values of allergen-specific IgE. This finding suggests that in most atopic patients, the quantity of irrelevant IgE plays a relatively minor role in determining allergic sensitivity to a given allergen. The implications of this finding are discussed.
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U2 - 10.1016/0091-6749(79)90156-8
DO - 10.1016/0091-6749(79)90156-8
M3 - Article
C2 - 82565
AN - SCOPUS:0018410016
SN - 0091-6749
VL - 63
SP - 15
EP - 22
JO - The Journal of allergy and clinical immunology
JF - The Journal of allergy and clinical immunology
IS - 1
ER -