Assessment of the androgen environment within the human testis: Minimally invasive method to obtain intratesticular fluid

J. P. Jarow, Haolin Chen, W. Rosner, S. Trentacoste, Barry R Zirkin

Research output: Contribution to journalArticle

Abstract

Previous studies of the rat have shown that testosterone concentrations within the interstitial and seminiferous tubular fluids of the testes are significantly higher than normal serum levels, and further, that although intratesticular testosterone concentration can be substantially reduced without an effect on spermatogenesis, the concentration that is minimally required to maintain spermatogenesis is also substantially higher than serum levels. The purpose of the present study was to adapt a minimally invasive technique to sample human intratesticular fluid to enable parallel observations in man. To this end, aspiration methods were first developed for the rat testis and then adapted to the human. The testosterone concentration in fluid obtained by unilateral aspiration of rat testes was approximately 50 ng/mL, similar to the known concentration in seminiferous tubular fluid. These aspiration methods were then adapted to obtain intratesticular fluid from human testes. Studies of 12 fertile human subjects demonstrated that percutaneous testicular aspiration could be performed safely and successfully using a 19-gauge needle. Nine additional human subjects had bilateral testicular aspiration and simultaneous measurement of peripheral blood testosterone levels. Testicular aspirations yielded 8 to 117 μL of fluid from each testicle. The mean concentration of testosterone in aspirates obtained from the 21 patients was 609 ± 50 ng/mL. Dihydrotestosterone and 3α-androstanediol concentrations were quite low, below the limits of detection of our assay. The SHBG/ABP concentration in the aspirates was 8.5 ± 1.1 nM. These results define testosterone as the major androgenic steroid in the human testis, as in the rat testis, and indicate that the testosterone concentration within the human testis is approximately 200-fold greater than that of SHBG/ABP, and more than 100-fold greater than the concentration of testosterone found in normal human serum.

Original languageEnglish (US)
Pages (from-to)640-645
Number of pages6
JournalJournal of Andrology
Volume22
Issue number4
StatePublished - 2001

Fingerprint

Androgens
Testosterone
Testis
Spermatogenesis
Serum
Dihydrotestosterone
Needles
Limit of Detection
Steroids

Keywords

  • ABP
  • Spermatogenesis
  • Testosterone

ASJC Scopus subject areas

  • Endocrinology

Cite this

Assessment of the androgen environment within the human testis : Minimally invasive method to obtain intratesticular fluid. / Jarow, J. P.; Chen, Haolin; Rosner, W.; Trentacoste, S.; Zirkin, Barry R.

In: Journal of Andrology, Vol. 22, No. 4, 2001, p. 640-645.

Research output: Contribution to journalArticle

@article{10d5312067ba47fdb072586db78495ce,
title = "Assessment of the androgen environment within the human testis: Minimally invasive method to obtain intratesticular fluid",
abstract = "Previous studies of the rat have shown that testosterone concentrations within the interstitial and seminiferous tubular fluids of the testes are significantly higher than normal serum levels, and further, that although intratesticular testosterone concentration can be substantially reduced without an effect on spermatogenesis, the concentration that is minimally required to maintain spermatogenesis is also substantially higher than serum levels. The purpose of the present study was to adapt a minimally invasive technique to sample human intratesticular fluid to enable parallel observations in man. To this end, aspiration methods were first developed for the rat testis and then adapted to the human. The testosterone concentration in fluid obtained by unilateral aspiration of rat testes was approximately 50 ng/mL, similar to the known concentration in seminiferous tubular fluid. These aspiration methods were then adapted to obtain intratesticular fluid from human testes. Studies of 12 fertile human subjects demonstrated that percutaneous testicular aspiration could be performed safely and successfully using a 19-gauge needle. Nine additional human subjects had bilateral testicular aspiration and simultaneous measurement of peripheral blood testosterone levels. Testicular aspirations yielded 8 to 117 μL of fluid from each testicle. The mean concentration of testosterone in aspirates obtained from the 21 patients was 609 ± 50 ng/mL. Dihydrotestosterone and 3α-androstanediol concentrations were quite low, below the limits of detection of our assay. The SHBG/ABP concentration in the aspirates was 8.5 ± 1.1 nM. These results define testosterone as the major androgenic steroid in the human testis, as in the rat testis, and indicate that the testosterone concentration within the human testis is approximately 200-fold greater than that of SHBG/ABP, and more than 100-fold greater than the concentration of testosterone found in normal human serum.",
keywords = "ABP, Spermatogenesis, Testosterone",
author = "Jarow, {J. P.} and Haolin Chen and W. Rosner and S. Trentacoste and Zirkin, {Barry R}",
year = "2001",
language = "English (US)",
volume = "22",
pages = "640--645",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "4",

}

TY - JOUR

T1 - Assessment of the androgen environment within the human testis

T2 - Minimally invasive method to obtain intratesticular fluid

AU - Jarow, J. P.

AU - Chen, Haolin

AU - Rosner, W.

AU - Trentacoste, S.

AU - Zirkin, Barry R

PY - 2001

Y1 - 2001

N2 - Previous studies of the rat have shown that testosterone concentrations within the interstitial and seminiferous tubular fluids of the testes are significantly higher than normal serum levels, and further, that although intratesticular testosterone concentration can be substantially reduced without an effect on spermatogenesis, the concentration that is minimally required to maintain spermatogenesis is also substantially higher than serum levels. The purpose of the present study was to adapt a minimally invasive technique to sample human intratesticular fluid to enable parallel observations in man. To this end, aspiration methods were first developed for the rat testis and then adapted to the human. The testosterone concentration in fluid obtained by unilateral aspiration of rat testes was approximately 50 ng/mL, similar to the known concentration in seminiferous tubular fluid. These aspiration methods were then adapted to obtain intratesticular fluid from human testes. Studies of 12 fertile human subjects demonstrated that percutaneous testicular aspiration could be performed safely and successfully using a 19-gauge needle. Nine additional human subjects had bilateral testicular aspiration and simultaneous measurement of peripheral blood testosterone levels. Testicular aspirations yielded 8 to 117 μL of fluid from each testicle. The mean concentration of testosterone in aspirates obtained from the 21 patients was 609 ± 50 ng/mL. Dihydrotestosterone and 3α-androstanediol concentrations were quite low, below the limits of detection of our assay. The SHBG/ABP concentration in the aspirates was 8.5 ± 1.1 nM. These results define testosterone as the major androgenic steroid in the human testis, as in the rat testis, and indicate that the testosterone concentration within the human testis is approximately 200-fold greater than that of SHBG/ABP, and more than 100-fold greater than the concentration of testosterone found in normal human serum.

AB - Previous studies of the rat have shown that testosterone concentrations within the interstitial and seminiferous tubular fluids of the testes are significantly higher than normal serum levels, and further, that although intratesticular testosterone concentration can be substantially reduced without an effect on spermatogenesis, the concentration that is minimally required to maintain spermatogenesis is also substantially higher than serum levels. The purpose of the present study was to adapt a minimally invasive technique to sample human intratesticular fluid to enable parallel observations in man. To this end, aspiration methods were first developed for the rat testis and then adapted to the human. The testosterone concentration in fluid obtained by unilateral aspiration of rat testes was approximately 50 ng/mL, similar to the known concentration in seminiferous tubular fluid. These aspiration methods were then adapted to obtain intratesticular fluid from human testes. Studies of 12 fertile human subjects demonstrated that percutaneous testicular aspiration could be performed safely and successfully using a 19-gauge needle. Nine additional human subjects had bilateral testicular aspiration and simultaneous measurement of peripheral blood testosterone levels. Testicular aspirations yielded 8 to 117 μL of fluid from each testicle. The mean concentration of testosterone in aspirates obtained from the 21 patients was 609 ± 50 ng/mL. Dihydrotestosterone and 3α-androstanediol concentrations were quite low, below the limits of detection of our assay. The SHBG/ABP concentration in the aspirates was 8.5 ± 1.1 nM. These results define testosterone as the major androgenic steroid in the human testis, as in the rat testis, and indicate that the testosterone concentration within the human testis is approximately 200-fold greater than that of SHBG/ABP, and more than 100-fold greater than the concentration of testosterone found in normal human serum.

KW - ABP

KW - Spermatogenesis

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=0034937154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034937154&partnerID=8YFLogxK

M3 - Article

C2 - 11451361

AN - SCOPUS:0034937154

VL - 22

SP - 640

EP - 645

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 4

ER -