Assessment of renal fibrosis in a rat model of unilateral ureteral obstruction with diffusion kurtosis imaging: Comparison with α-SMA expression and 18F-FDG PET

Anqin Li, Lili Liang, Ping Liang, Yao Hu, Chuou Xu, Xuemei Hu, Yaqi Shen, Daoyu Hu, Zhen Li, Ihab R. Kamel

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To investigate the utility of diffusion kurtosis imaging (DKI) MRI for evaluation of renal fibrosis in rats with unilateral ureteral obstruction (UUO). Methods: Twenty-five rats had UUO, and ten rats were subjected to sham operation as control. DKI was performed on a 3.0 T MRI scanner on days 1, 3, 5, and 7 after ligation. All rats then underwent 18F-FDG dynamic PET to evaluate unilateral renal function, followed by histological analysis to examine α-smooth muscle actin (α-SMA) expression. DKI metrics were assessed among the time points and between two sides, and compared with maximum standardized uptake value (SUVmax), serum levels of creatinine and urea, and fibrosis marker α-SMA. Results: Mean kurtosis (MK) on day 7, axial kurtosis (Ka) on days 3 and 7, mean diffusivity (MD) on days 1, 3, 5, and 7, and fractional anisotropy (FA) on days 3, 5, and 7 of cortex and medulla between the UUO and contralateral sides were significantly different (all p < 0.05). Over the course of UUO progression, there were significant changes in Ka, MD and FA of medulla (all p < 0.05). FA of medulla was positively correlated with SUVmax (r = 0.641, p < 0.001), and MD of cortex was negatively correlated with urea (r = −0.534, p = 0.001). MD of cortex was negatively correlated with α-SMA on UUO sides (r = −0.710, p < 0.001). Conclusions: DKI shows the potential for noninvasive assessment of renal fibrosis and unilateral renal function induced by UUO.

Original languageEnglish (US)
Pages (from-to)176-184
Number of pages9
JournalMagnetic Resonance Imaging
Volume66
DOIs
StatePublished - Feb 2020

Keywords

  • Diffusion kurtosis imaging
  • Magnetic resonance imaging
  • Renal fibrosis
  • Unilateral ureteral obstruction

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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