TY - JOUR
T1 - Assessment of renal fibrosis in a rat model of unilateral ureteral obstruction with diffusion kurtosis imaging
T2 - Comparison with α-SMA expression and 18F-FDG PET
AU - Li, Anqin
AU - Liang, Lili
AU - Liang, Ping
AU - Hu, Yao
AU - Xu, Chuou
AU - Hu, Xuemei
AU - Shen, Yaqi
AU - Hu, Daoyu
AU - Li, Zhen
AU - Kamel, Ihab R.
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China [grant numbers 81771801 , 81571642 , 81701657 and 81801695 ].
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2020/2
Y1 - 2020/2
N2 - Purpose: To investigate the utility of diffusion kurtosis imaging (DKI) MRI for evaluation of renal fibrosis in rats with unilateral ureteral obstruction (UUO). Methods: Twenty-five rats had UUO, and ten rats were subjected to sham operation as control. DKI was performed on a 3.0 T MRI scanner on days 1, 3, 5, and 7 after ligation. All rats then underwent 18F-FDG dynamic PET to evaluate unilateral renal function, followed by histological analysis to examine α-smooth muscle actin (α-SMA) expression. DKI metrics were assessed among the time points and between two sides, and compared with maximum standardized uptake value (SUVmax), serum levels of creatinine and urea, and fibrosis marker α-SMA. Results: Mean kurtosis (MK) on day 7, axial kurtosis (Ka) on days 3 and 7, mean diffusivity (MD) on days 1, 3, 5, and 7, and fractional anisotropy (FA) on days 3, 5, and 7 of cortex and medulla between the UUO and contralateral sides were significantly different (all p < 0.05). Over the course of UUO progression, there were significant changes in Ka, MD and FA of medulla (all p < 0.05). FA of medulla was positively correlated with SUVmax (r = 0.641, p < 0.001), and MD of cortex was negatively correlated with urea (r = −0.534, p = 0.001). MD of cortex was negatively correlated with α-SMA on UUO sides (r = −0.710, p < 0.001). Conclusions: DKI shows the potential for noninvasive assessment of renal fibrosis and unilateral renal function induced by UUO.
AB - Purpose: To investigate the utility of diffusion kurtosis imaging (DKI) MRI for evaluation of renal fibrosis in rats with unilateral ureteral obstruction (UUO). Methods: Twenty-five rats had UUO, and ten rats were subjected to sham operation as control. DKI was performed on a 3.0 T MRI scanner on days 1, 3, 5, and 7 after ligation. All rats then underwent 18F-FDG dynamic PET to evaluate unilateral renal function, followed by histological analysis to examine α-smooth muscle actin (α-SMA) expression. DKI metrics were assessed among the time points and between two sides, and compared with maximum standardized uptake value (SUVmax), serum levels of creatinine and urea, and fibrosis marker α-SMA. Results: Mean kurtosis (MK) on day 7, axial kurtosis (Ka) on days 3 and 7, mean diffusivity (MD) on days 1, 3, 5, and 7, and fractional anisotropy (FA) on days 3, 5, and 7 of cortex and medulla between the UUO and contralateral sides were significantly different (all p < 0.05). Over the course of UUO progression, there were significant changes in Ka, MD and FA of medulla (all p < 0.05). FA of medulla was positively correlated with SUVmax (r = 0.641, p < 0.001), and MD of cortex was negatively correlated with urea (r = −0.534, p = 0.001). MD of cortex was negatively correlated with α-SMA on UUO sides (r = −0.710, p < 0.001). Conclusions: DKI shows the potential for noninvasive assessment of renal fibrosis and unilateral renal function induced by UUO.
KW - Diffusion kurtosis imaging
KW - Magnetic resonance imaging
KW - Renal fibrosis
KW - Unilateral ureteral obstruction
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U2 - 10.1016/j.mri.2019.08.035
DO - 10.1016/j.mri.2019.08.035
M3 - Article
C2 - 31484043
AN - SCOPUS:85071927537
SN - 0730-725X
VL - 66
SP - 176
EP - 184
JO - Magnetic Resonance Imaging
JF - Magnetic Resonance Imaging
ER -