Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy

Yu Hang Liu, Lun De Liao, Stacey Sze Hui Tan, Ki Yong Kwon, Ji Min Ling, Aishwarya Bandla, Yen Yu Ian Shih, Eddie Tung Wee Tan, Wen Li, Wai Hoe Ng, Hsin Yi Lai, You Yin Chen, Nitish V Thakor

Research output: Contribution to journalArticle

Abstract

This study developed a novel system combining a 16-channel micro-electrocorticography (μECoG) electrode array and functional photoacoustic microscopy (fPAM) to examine changes in neurovascular functions following transient ischemic attack (TIA) in rats. To mimic the pathophysiology of TIA, a modified photothrombotic ischemic model was developed by using 3min illumination of 5mW continuous-wave (CW) green laser light focusing on a distal branch of the middle cerebral artery (MCA). Cerebral blood volume (CBV), hemoglobin oxygen saturation (SO2), somatosensory evoked potentials (SSEPs) and alpha-to-delta ratio (ADR) were measured pre- and post-ischemia over a focal cortical region (i.e., 1.5×1.5mm2). Unexpectedly, the SO2, peak-to-peak amplitude (PPA) of SSEPs and ADR recovered and achieved levels greater than the baseline values at the 4th hour post-ischemia induction without any intervention, whereas the CBV value only partially recovered. In other words, transient ischemia led to increased neural activity when the relative CBV was reduced, which may further compromise neural integrity or lead to subsequent vascular disease. This novel μECoG-fPAM system complements currently available imaging techniques and represents a promising technology for studying neurovascular coupling in animal models.

Original languageEnglish (US)
Pages (from-to)455-465
Number of pages11
JournalNeurobiology of Disease
Volume82
DOIs
StatePublished - Oct 1 2015

Fingerprint

Transient Ischemic Attack
Microscopy
Electrodes
Ischemia
Somatosensory Evoked Potentials
Methyl Green
Middle Cerebral Artery
Lighting
Vascular Diseases
Hemoglobins
Lasers
Animal Models
Oxygen
Technology
Electrocorticography
Cerebral Blood Volume

Keywords

  • Functional photoacoustic microscopy (fPAM)
  • Micro-electrocorticography (μECoG)
  • Neurovascular function
  • Photothrombotic ischemia
  • Transient ischemic attack (TIA)

ASJC Scopus subject areas

  • Neurology

Cite this

Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy. / Liu, Yu Hang; Liao, Lun De; Tan, Stacey Sze Hui; Kwon, Ki Yong; Ling, Ji Min; Bandla, Aishwarya; Shih, Yen Yu Ian; Tan, Eddie Tung Wee; Li, Wen; Ng, Wai Hoe; Lai, Hsin Yi; Chen, You Yin; Thakor, Nitish V.

In: Neurobiology of Disease, Vol. 82, 01.10.2015, p. 455-465.

Research output: Contribution to journalArticle

Liu, Yu Hang ; Liao, Lun De ; Tan, Stacey Sze Hui ; Kwon, Ki Yong ; Ling, Ji Min ; Bandla, Aishwarya ; Shih, Yen Yu Ian ; Tan, Eddie Tung Wee ; Li, Wen ; Ng, Wai Hoe ; Lai, Hsin Yi ; Chen, You Yin ; Thakor, Nitish V. / Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy. In: Neurobiology of Disease. 2015 ; Vol. 82. pp. 455-465.
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AU - Liu, Yu Hang

AU - Liao, Lun De

AU - Tan, Stacey Sze Hui

AU - Kwon, Ki Yong

AU - Ling, Ji Min

AU - Bandla, Aishwarya

AU - Shih, Yen Yu Ian

AU - Tan, Eddie Tung Wee

AU - Li, Wen

AU - Ng, Wai Hoe

AU - Lai, Hsin Yi

AU - Chen, You Yin

AU - Thakor, Nitish V

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N2 - This study developed a novel system combining a 16-channel micro-electrocorticography (μECoG) electrode array and functional photoacoustic microscopy (fPAM) to examine changes in neurovascular functions following transient ischemic attack (TIA) in rats. To mimic the pathophysiology of TIA, a modified photothrombotic ischemic model was developed by using 3min illumination of 5mW continuous-wave (CW) green laser light focusing on a distal branch of the middle cerebral artery (MCA). Cerebral blood volume (CBV), hemoglobin oxygen saturation (SO2), somatosensory evoked potentials (SSEPs) and alpha-to-delta ratio (ADR) were measured pre- and post-ischemia over a focal cortical region (i.e., 1.5×1.5mm2). Unexpectedly, the SO2, peak-to-peak amplitude (PPA) of SSEPs and ADR recovered and achieved levels greater than the baseline values at the 4th hour post-ischemia induction without any intervention, whereas the CBV value only partially recovered. In other words, transient ischemia led to increased neural activity when the relative CBV was reduced, which may further compromise neural integrity or lead to subsequent vascular disease. This novel μECoG-fPAM system complements currently available imaging techniques and represents a promising technology for studying neurovascular coupling in animal models.

AB - This study developed a novel system combining a 16-channel micro-electrocorticography (μECoG) electrode array and functional photoacoustic microscopy (fPAM) to examine changes in neurovascular functions following transient ischemic attack (TIA) in rats. To mimic the pathophysiology of TIA, a modified photothrombotic ischemic model was developed by using 3min illumination of 5mW continuous-wave (CW) green laser light focusing on a distal branch of the middle cerebral artery (MCA). Cerebral blood volume (CBV), hemoglobin oxygen saturation (SO2), somatosensory evoked potentials (SSEPs) and alpha-to-delta ratio (ADR) were measured pre- and post-ischemia over a focal cortical region (i.e., 1.5×1.5mm2). Unexpectedly, the SO2, peak-to-peak amplitude (PPA) of SSEPs and ADR recovered and achieved levels greater than the baseline values at the 4th hour post-ischemia induction without any intervention, whereas the CBV value only partially recovered. In other words, transient ischemia led to increased neural activity when the relative CBV was reduced, which may further compromise neural integrity or lead to subsequent vascular disease. This novel μECoG-fPAM system complements currently available imaging techniques and represents a promising technology for studying neurovascular coupling in animal models.

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