TY - JOUR
T1 - Assessment of Neural Cell Adhesion Molecule (NCAM) in Autistic Serum and Postmortem Brain
AU - Purcell, Amy E.
AU - Rocco, Mary Margaret
AU - Lenhart, Jennifer A.
AU - Hyder, Karim
AU - Zimmerman, Andrew W.
AU - Pevsner, Jonathan
N1 - Funding Information:
The authors thank Audrius V. Plioplys for support. For providing the autistic clinical data for the post-mortem brain samples we also thank Susan Folstein, Margaret Bauman, Anne Booker, and Julia DiBello. The authors are also grateful for the help of Scott Zeger and Elizabeth Johnson (Department of Biostatistics, Johns Hopkins School of Hygiene and Public Health, Baltimore) with data analysis and Alex Chenchik (CLONTECH Laboratories) for support. We thank the brain banks, including the Autism Research Foundation (TARF), for providing postmortem brain tissue. The Harvard Brain Tissue Resource Center is supported by PHS Grant MH/NS 31862. The University of Miami/ University of Maryland Brain and Tissue Bank operates under NICHD contract #N01-HD-8-3284. The authors thank Justin C. McArthur and Philip Feaser (Department of Neurology, Johns Hopkins University) for generously providing serum from control individuals. Their work is supported by Grant N526643 (to J.C.M.). The data analysis was supported by the MRDDRC. A.E.P. is supported by a National Science Foundation predoctoral training grant. A grant from Solving the Mystery of Autism (to J. P.) helped fund the Incyte Genomics microarray research. This project was supported by a grant from the National Alliance for Autism Research (NAAR: to A. Z. and J. P.).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2001/4
Y1 - 2001/4
N2 - Studies have identified structural abnormalities in areas of the autistic brain, with a pattern suggesting that a neurodevelopmental anomaly took place. Neural cell adhesion molecule (NCAM), which is involved in development of the central nervous system, was previously shown to be decreased in the serum of autistic individuals. In the present study, we measured NCAM protein in the sera from controls, patients with autism, siblings of autistic patients, and individuals with other neurologic disorders, but found no significant differences. We also measured NCAM protein in autistic postmortem brain samples and found the longest isoform, NCAM-180, to be significantly decreased. In addition, we investigated the mRNA expression of NCAM in these brain samples using cDNA microarrays and RT-PCR. Results show that NCAM mRNA levels are not altered in autism.
AB - Studies have identified structural abnormalities in areas of the autistic brain, with a pattern suggesting that a neurodevelopmental anomaly took place. Neural cell adhesion molecule (NCAM), which is involved in development of the central nervous system, was previously shown to be decreased in the serum of autistic individuals. In the present study, we measured NCAM protein in the sera from controls, patients with autism, siblings of autistic patients, and individuals with other neurologic disorders, but found no significant differences. We also measured NCAM protein in autistic postmortem brain samples and found the longest isoform, NCAM-180, to be significantly decreased. In addition, we investigated the mRNA expression of NCAM in these brain samples using cDNA microarrays and RT-PCR. Results show that NCAM mRNA levels are not altered in autism.
KW - Autism
KW - Brain
KW - Neural cell adhesion molecule
UR - http://www.scopus.com/inward/record.url?scp=0035317628&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035317628&partnerID=8YFLogxK
U2 - 10.1023/A:1010751232295
DO - 10.1023/A:1010751232295
M3 - Article
C2 - 11450817
AN - SCOPUS:0035317628
VL - 31
SP - 183
EP - 194
JO - Journal of Autism and Developmental Disorders
JF - Journal of Autism and Developmental Disorders
SN - 0162-3257
IS - 2
ER -