Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS

Guido Massaccesi, Michael A. Chattergoon

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The innate immune system is an evolutionarily conserved host defense system and is the first barrier to infection. The system utilizes genetically conserved receptors to identify the presence of microbial structures. Engagement of innate immune receptors by primarily by ligands that discriminate pathogens from the host activates programmed responses that limit pathogen expansion. Despite its ubiquitous nature, surprisingly DNA is a critical structure that triggers innate immune responses. Focusing on structural modifications or aberrant location of DNA, innate immune receptors identify physiologic stress. Inflammasomes and interferons are critical innate immune pathways that are activated by DNA. DNA binding proteins that tie recognition of DNA to both programmed responses have been identified, and their importance demonstrated in infection models. In this chapter, we discuss techniques to analyze AIM2 inflammasome and cGAS interferon activation by synthetic DNA and DNA viruses. We also discuss methods to measure the activity of these immune pathways.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsJungsan Sohn
PublisherAcademic Press Inc.
Pages269-285
Number of pages17
ISBN (Print)9780128183595
DOIs
StatePublished - 2019

Publication series

NameMethods in Enzymology
Volume625
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • AIM2
  • DNA PAMP
  • IL-18
  • Inflammasome
  • Type I interferon
  • cGAS

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Massaccesi, G., & Chattergoon, M. A. (2019). Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS. In J. Sohn (Ed.), Methods in Enzymology (pp. 269-285). (Methods in Enzymology; Vol. 625). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2019.05.008