Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR

Xin Jiang; Wenhong Zhang; Ying Zhang; Feng Gao; Chanyi Lu; Xuelian Zhang; Honghai Wang

doi:10.1089/mdr.2008.0772

Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR

Xin Jiang, Wenhong Zhang, Ying Zhang, Feng Gao, Chanyi Lu, Xuelian Zhang, Honghai Wang

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p < 0.05). Overexpression of some efflux genes is associated with multidrug resistance in certain clinical isolate of M. tuberculosis.

Original language	English (US)
Pages (from-to)	7-11
Number of pages	5
Journal	Microbial Drug Resistance
Volume	14
Issue number	1
DOIs	https://doi.org/10.1089/mdr.2008.0772
State	Published - Mar 1 2008
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Pharmacology
Microbiology (medical)

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abstract = "The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p < 0.05). Overexpression of some efflux genes is associated with multidrug resistance in certain clinical isolate of M. tuberculosis.",

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AU - Zhang, Wenhong

AU - Zhang, Ying

AU - Gao, Feng

AU - Lu, Chanyi

AU - Zhang, Xuelian

AU - Wang, Honghai

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AB - The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p < 0.05). Overexpression of some efflux genes is associated with multidrug resistance in certain clinical isolate of M. tuberculosis.

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Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR

Abstract

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