Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [125I]-4-iododexetimide and [125I]-4-iodolevetimide

Sasaki Masayuki; Hans W. Müller-Gärtner; John R. Lever; Hayden T. Ravert; Robert F. Dannals; Tomas R. Guilarte; Henry N. Wagner

doi:10.1016/0028-3908(93)90043-3

Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [¹²⁵I]-4-iododexetimide and [¹²⁵I]-4-iodolevetimide

Sasaki Masayuki, Hans W. Müller-Gärtner, John R. Lever, Hayden T. Ravert, Robert F. Dannals, Tomas R. Guilarte, Henry N. Wagner

School of Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [¹²⁵I]-4-iododexetimide, and the inactive enantiomer [¹²⁵I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.

Original language	English (US)
Pages (from-to)	1441-1443
Number of pages	3
Journal	Neuropharmacology
Volume	32
Issue number	12
DOIs	https://doi.org/10.1016/0028-3908(93)90043-3
State	Published - Dec 1993

Keywords

4-iododexetimide
4-iodolevetimide
muscarinic acetylcholinergic receptor
radioiodine
single photon detection system

ASJC Scopus subject areas

Pharmacology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0028-3908(93)90043-3

Cite this

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title = "Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [125I]-4-iododexetimide and [125I]-4-iodolevetimide",

abstract = "This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [125I]-4-iododexetimide, and the inactive enantiomer [125I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.",

keywords = "4-iododexetimide, 4-iodolevetimide, muscarinic acetylcholinergic receptor, radioiodine, single photon detection system",

author = "Sasaki Masayuki and M{\"u}ller-G{\"a}rtner, {Hans W.} and Lever, {John R.} and Ravert, {Hayden T.} and Dannals, {Robert F.} and Guilarte, {Tomas R.} and Wagner, {Henry N.}",

year = "1993",

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doi = "10.1016/0028-3908(93)90043-3",

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journal = "Neuropharmacology",

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AU - Masayuki, Sasaki

AU - Müller-Gärtner, Hans W.

AU - Lever, John R.

AU - Ravert, Hayden T.

AU - Dannals, Robert F.

AU - Guilarte, Tomas R.

AU - Wagner, Henry N.

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AB - This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [125I]-4-iododexetimide, and the inactive enantiomer [125I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.

KW - 4-iododexetimide

KW - 4-iodolevetimide

KW - muscarinic acetylcholinergic receptor

KW - radioiodine

KW - single photon detection system

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