Assessment of brain muscarinic acetylcholinergic receptors in living mice using a simple probe, [125I]-4-iododexetimide and [125I]-4-iodolevetimide

Sasaki Masayuki, Hans W. Müller-Gärtner, John R. Lever, Hayden T. Ravert, Robert F. Dannals, Tomas R. Guilarte, Henry N. Wagner

Research output: Contribution to journalArticle

Abstract

This study describes assessment of brain muscarinic acetylcholinergic receptors in living mice using a single-crystal radiation detection system, the high-affinity antagonist [125I]-4-iododexetimide, and the inactive enantiomer [125I]-4-iodolevetimide. Kinetics of radioligand binding, as well as perturbation by atropine displacement, can be determined using this simple probe technique.

Original languageEnglish (US)
Pages (from-to)1441-1443
Number of pages3
JournalNeuropharmacology
Volume32
Issue number12
DOIs
StatePublished - Dec 1993

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Keywords

  • 4-iododexetimide
  • 4-iodolevetimide
  • muscarinic acetylcholinergic receptor
  • radioiodine
  • single photon detection system

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

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