Assessing Reality Testing in Mice Through Dopamine-Dependent Associatively Evoked Processing of Absent Gustatory Stimuli

Benjamin R. Fry, Nicollette Russell, Ryan Gifford, Cindee F. Robles, Claire E. Manning, Akira Sawa, Minae Niwa, Alexander W. Johnson

Research output: Contribution to journalArticle

Abstract

Impairments in reality testing are core features of numerous neuropsychiatric conditions. However, relatively few animal models have been developed to assess this critical facet of neuropsychiatric illness, thus impeding our understanding of the underlying central systems and circuits. Using mice in which dominant-negative Disrupted-in-Schizophrenia-1 is expressed throughout central nervous system circuitry (DN-DISC1-PrP), the capacity for an auditory conditioned stimulus (CS) to evoke perceptual processing of an absent sucrose solution was examined. At test, during CS presentations, DN-DISC1-PrP mice consumed more water and displayed a licking profile that is more typically revealed while ingesting a sweet-tasting solution. DN-DISC1-PrP mice also displayed greater c-fos expression in the insular (gustatory) cortex when consuming water in the presence of the CS. This capacity for the CS to more readily substitute for the taste features of the absent sucrose solution in DN-DISC1-PrP mice was attenuated following systemic treatment with the antipsychotic haloperidol. Conversely, social isolation during adolescence promoted the manifestation of these effects. These results provide strong validation for using associative learning procedures to examine dopamine-mediated reality testing associated with insular cortex activation.

Original languageEnglish (US)
Pages (from-to)54-67
Number of pages14
JournalSchizophrenia bulletin
Volume46
Issue number1
DOIs
StatePublished - Jan 4 2020

Fingerprint

Reality Testing
Dopamine
Cerebral Cortex
Sucrose
Social Isolation
Water
Haloperidol
Antipsychotic Agents
Schizophrenia
Central Nervous System
Animal Models
Learning

Keywords

  • delusions
  • hallucinations
  • neuroleptics
  • neuropsy- chiatric illness
  • reward

ASJC Scopus subject areas

  • Psychiatry and Mental health

Cite this

Assessing Reality Testing in Mice Through Dopamine-Dependent Associatively Evoked Processing of Absent Gustatory Stimuli. / Fry, Benjamin R.; Russell, Nicollette; Gifford, Ryan; Robles, Cindee F.; Manning, Claire E.; Sawa, Akira; Niwa, Minae; Johnson, Alexander W.

In: Schizophrenia bulletin, Vol. 46, No. 1, 04.01.2020, p. 54-67.

Research output: Contribution to journalArticle

Fry, Benjamin R. ; Russell, Nicollette ; Gifford, Ryan ; Robles, Cindee F. ; Manning, Claire E. ; Sawa, Akira ; Niwa, Minae ; Johnson, Alexander W. / Assessing Reality Testing in Mice Through Dopamine-Dependent Associatively Evoked Processing of Absent Gustatory Stimuli. In: Schizophrenia bulletin. 2020 ; Vol. 46, No. 1. pp. 54-67.
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