TY - JOUR
T1 - Assessing basophil functional measures during monoclonal anti-IgE therapy
AU - Saini, Sarbjit S.
AU - MacGlashan, Donald W.
PY - 2012/9/28
Y1 - 2012/9/28
N2 - Assessing the impact of therapeutic interventions on the clinical and immunologic responses of allergic subjects is a topic of extensive investigation. Available approaches include the measurement of in vivo allergen challenge responses, serologic measures, or in vitro studies of cells that participate in the allergic reaction. Several decades of work support that measures of allergen responses of IgE-bearing peripheral blood basophils can reflect clinical expression of allergic disease. In the last decade, an immune-based therapy targeting IgE, omalizumab, has emerged as an adjunct treatment for a variety of allergic diseases. This monoclonal humanized IgG antibody specifically binds circulating IgE at a region in the Fc tail that prevents IgE attachment to high affinity IgE receptor (FcεRI) bearing cell types such as tissue mast cells and blood basophils. This review focuses on methods to monitor changes of basophil allergen reactivity with a focus on omalizumab therapy and the implications for clinical disease management.
AB - Assessing the impact of therapeutic interventions on the clinical and immunologic responses of allergic subjects is a topic of extensive investigation. Available approaches include the measurement of in vivo allergen challenge responses, serologic measures, or in vitro studies of cells that participate in the allergic reaction. Several decades of work support that measures of allergen responses of IgE-bearing peripheral blood basophils can reflect clinical expression of allergic disease. In the last decade, an immune-based therapy targeting IgE, omalizumab, has emerged as an adjunct treatment for a variety of allergic diseases. This monoclonal humanized IgG antibody specifically binds circulating IgE at a region in the Fc tail that prevents IgE attachment to high affinity IgE receptor (FcεRI) bearing cell types such as tissue mast cells and blood basophils. This review focuses on methods to monitor changes of basophil allergen reactivity with a focus on omalizumab therapy and the implications for clinical disease management.
KW - Basophils
KW - IgE
KW - Omalizumab
UR - http://www.scopus.com/inward/record.url?scp=84864384389&partnerID=8YFLogxK
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U2 - 10.1016/j.jim.2012.05.016
DO - 10.1016/j.jim.2012.05.016
M3 - Review article
C2 - 22664098
AN - SCOPUS:84864384389
SN - 0022-1759
VL - 383
SP - 60
EP - 64
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -