Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set

Vincent VanBuren, Yulan Piao, Dawood B. Dudekula, Yong Qian, Carter G. Carter, Patrick R. Martin, Carole A. Stagg, Uwem C. Bassey, Kazuhiro Aiba, Toshio Hamatani, George J. Kargul, Amber G. Luo, Janet Kelso, Winston Hide, Minoru S.H. Ko

Research output: Contribution to journalReview article

Abstract

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.

Original languageEnglish (US)
Pages (from-to)1999-2003
Number of pages5
JournalGenome research
Volume12
Issue number12
DOIs
StatePublished - Dec 1 2002

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    VanBuren, V., Piao, Y., Dudekula, D. B., Qian, Y., Carter, C. G., Martin, P. R., Stagg, C. A., Bassey, U. C., Aiba, K., Hamatani, T., Kargul, G. J., Luo, A. G., Kelso, J., Hide, W., & Ko, M. S. H. (2002). Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set. Genome research, 12(12), 1999-2003. https://doi.org/10.1101/gr.633802